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QUESTIVIES You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid (PUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7031.1 H FOXP2 H H XRAY H H <--300kb- H-Hind!!! 1500bp 600bp 900bp Size Colony Colony Colony Colony Colony Colony Standard 1 3 C 2kb - 1kb 200bp Insert Check-Gel of your samples n Wells MCS PUC-123 ori amp How large is your plasmid? O a. 1500bp O b. 1700bp O c. 900bp O d. 600bp O e. 2kb Click Save and Submit to save and submit. Click Save All Answers to save all answers.

QUESTIVIES You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid (PUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a 'insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7031.1 H FOXP2 H H XRAY H H <--300kb- H-Hind!!! 1500bp 600bp 900bp Size Colony Colony Colony Colony Colony Colony Standard 1 3 C 2kb - 1kb 200bp Insert Check-Gel of your samples n Wells MCS PUC-123 ori amp How large is your plasmid? O a. 1500bp O b. 1700bp O c. 900bp O d. 600bp O e. 2kb Click Save and Submit to save and submit. Click Save All Answers to save all answers.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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QUESTIONS You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid (PUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a "insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7031.1 H FOXP2 H H XRAY H H <--300kb- H-Hind!!! 1500bp 600bp 900bp Size Colony Colony Colony Colony Colony Colony Standard 1 3 - 2kb - 1kb 200bp Insert Check-Gel of your samples m M Wells Handill MCS PUC-123 ori amp How large is your plasmid? O a. 1500bp O b. 1700bp O c. 900bp O d. 600bp O e. 2kb Click Save and Submit to save and submit. Click Save All Answers to save all answers.
Transcribed Image Text:QUESTIONS You are studying a group of individuals with X-ray vision and perform linkage analysis and note that the trait seems to be linked to a variant of the FOXP2 gene. You have identified the minimum tiling path around FOXP2 and sequence all the clones. After assembling the sequences into a contiguous sequence, you examine it for any other protein coding genes. You identify 5 genes in this area that could be responsible for x-ray vision. You identify one of the five genes as the best candidate for x-ray vision and call it XRAY (partial map below). You want to isolate the gene in a plasmid so you digest your genomic DNA with the staggered-end cutting restriction enzyme Hindill and clone the digested DNA into your plasmid (PUC123) (map below). You pick 6 bacterial colonies, grow them and isolate the plasmids. You perform a "insert check' to determine which bacterial colonies have a plasmid containing the Xray gene (gel image below). Below is the map of the XRAY region with restriction sites indicated. Use these figures to answer the following question. Chromosome 7031.1 H FOXP2 H H XRAY H H <--300kb- H-Hind!!! 1500bp 600bp 900bp Size Colony Colony Colony Colony Colony Colony Standard 1 3 - 2kb - 1kb 200bp Insert Check-Gel of your samples m M Wells Handill MCS PUC-123 ori amp How large is your plasmid? O a. 1500bp O b. 1700bp O c. 900bp O d. 600bp O e. 2kb Click Save and Submit to save and submit. Click Save All Answers to save all answers.
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