Matching Match each item with the correct statement below. Not all terms will be used a. 5' GTP cap f. RFLPs b. Target copy g. sticky ends c. Ligase h. cloned DNA d. Cas-9 i recombinant DNA j. ampicillin e. Host cell 1. A cell (usually a bacteria) that has a gene put in it so the gene can be cloned 2. An example of a post-transcriptional modification of mRNA 3. Sections of DNA that vary between individuals. These can be used for DNA fingerprinting 4. May be used following transformation to kill off cells that a gene didn't enter 5. Molecule of DNA created during PCR that is the desired length 6. A molecule of DNA that comes from two different sources that are spliced together 7. Ends of DNA cut by a restriction enzyme where not all nucleotides are paired (i.e. ends are uneven) 8. Protein that works with CRISPR to cut DNA at a specific spot

Matching Match each item with the correct statement below. Not all terms will be used a. 5' GTP cap f. RFLPs b. Target copy g. sticky ends c. Ligase h. cloned DNA d. Cas-9 i recombinant DNA j. ampicillin e. Host cell 1. A cell (usually a bacteria) that has a gene put in it so the gene can be cloned 2. An example of a post-transcriptional modification of mRNA 3. Sections of DNA that vary between individuals. These can be used for DNA fingerprinting 4. May be used following transformation to kill off cells that a gene didn't enter 5. Molecule of DNA created during PCR that is the desired length 6. A molecule of DNA that comes from two different sources that are spliced together 7. Ends of DNA cut by a restriction enzyme where not all nucleotides are paired (i.e. ends are uneven) 8. Protein that works with CRISPR to cut DNA at a specific spot

Name: MatchingMatch each item with the correct statement below. Not all ter
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Description: MatchingMatch each item with the correct statement below. Not all terms will be useda. 5' GTP capf. RFLPsb. Target copyg. sticky endsc. Ligaseh. cloned DNAd. Cas-9i recombinant DNAj. ampicilline. Host cell1. A cell (usually a bacteria) that has a ge

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter10: Control Of Gene Expression

Section: Chapter Questions

Problem 2SQ: Binding or ___ to ___ in DNA can increase the rate of transcription of specific genes. a....

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magine you are working in a lab that is developing a novelty rose that smells like a stinkhorn mushroom – called the “Grose.” The gene that produces the odorous protein has been isolated from stinkhorn and recombinant DNA containing this gene, a promoter, and a gene that confers resistance to the antibiotic ampicillin has already been produced.Explain two methods you could use to introduce the recombinant DNA into rose cells. (ie. transforming the rose cells)
The work ‘Hybridization’ in DNA finger printing meansa) Pairing between the nucleotides of DNA sample with probeb) Pairing between the nucleotides of DNA and mRNAc) Pairing between the nucleotides of probe with mRNAd) Pairing between the nucleosides with mRNA
Cas9 is a protein that cuts nucleic acids a delivery vector a small DNA binding protein clusters of short palindromic DNA
Bacteriophage λ, after infecting a cell, can integrateinto the chromosome of the cell if the repressor protein, cI, binds to and shuts down phage transcriptionimmediately. (A strain containing a bacteriophageDNA integrated into the chromosome is called a lysogen.) The alternative fate is the production of manymore viruses and lysis of the cell. In a mating, a donor strain that is a lysogen was crossed with a lysogenic recipient cell, and no phages were produced.However, when the lysogen donor strain transferredits DNA to a nonlysogenic recipient cell, the recipientcell burst, releasing a new generation of phages. a. Why did the mating with a nonlysogenic recipientresult in phage growth and release, but the infectionof a lysogenic recipient did not?
Matching type Choices are in the picture 6. RF1 and RF2 recognize the three bases to terminate the process 7. CAAT box is found 80 nucleotides from the actual site 8. rho factor and sequence of uracil in a loop conclude the process 9. sigma factor binds to RNA polymerase in the promoter region 10. methionine is removed
In gene therapy, why can't a gene be inserted directly into the cell? It was mentioned on this website: https://ghr.nlm.nih.gov/primer/therapy/procedures and many others but there's no explanation for it.
Supercoiling of DNA requires GTP as source of energy is only observed in prokaryotes is not observed in eukaryotes requires the action of topoisomerases
Put these events in chronological order and explain why (i.e. each process listed). Sigma factor binds to RNA polymerase. Sigma factor binds to the promoter region. RNA synthesis begins. The double helix of DNA is unwound, breaking apart the complementary strands. Sigma factor is released.
Need help Which of the following is NOT a step in the formation of LTRs? A. Binding of a tRNA primer to the primer binding site sequences on the retroviral RNA genome B. Synthesis of a DNA segment starting from a primer hybridized to the RNA poylpurine tract by DNA polymerase C. A jump in which an R segment (DNA) hybridizes to an (RNA) R segment on the 3' end of the retroviral RNA genome D. Degradation of an RNA strand by RNase H that stops at the primer binding site E. Circularization by annealing of the single-stranded primer binding sites (PBS) to one another
Multiple Matching. Fill in the blanks with the words below that apply.18. ________ site of protein synthesis19. ________ carries the codon20. ________ carries the anticodon21. ________ a process synonymous with mRNA synthesis22. ________ bacteriophages participate in this transfer23. ________ duplication of the DNA molecule24. ________ process in which transcribed DNA code is deciphered into a polypeptide25. ________ involves plasmidsreplicationtRNAconjugationribosometransductionmRNAtranscriptiontransformationtranslationnone of these PreviousNext
True or False? LINE elements encode functional transposition protein and thus can move autonomously in the human genome. True or False? Target-primed reverse transcription can mobilize information through an RNA intermediate.
The function of ligase is toa. rejoin segments of DNAb. make longitudinal cuts in DNAc. synthesize cDNAd. break down ligaments