Recombinant bacteria can produce hormones that are normally produced in humans. Briefly describe how this is accomplished.

Q: Insulin produced by molecular cloning is ________. of pig origin of pig origin made by the human…

A: Introduction :- Pancreas is both an endocrine, as well as the exocrine gland. In its endocrine…

Q: RT-PCR can determine the amount of mRNA for a particular gene in two different growth conditions.

A: Reverse transcriptase polymerase chain reaction (RT-PCR) is a laboratory technique combining…

Q: Why must the gene be inserted into a vector for it to be cloned?

A: Cloning is the process by which a foreign DNA is inserted into the host cell for the purpose of…

Q: Briefly explain how bacteriophages can transduce genes between bacteria

A: Introduction :- Bacteriophages (BPs) are bacteria-infecting viruses that kill bacteria without…

Q: Name any two diseases for which transgenic mice are used as model organisms.

A: The transgenic mouse is obtained by the introduction of foreign DNA into the genome of the mouse. It…

Q: what are the advantages of using recombinant dna to produce human hormones such as insulin and…

A: The recomponent DNA technological processes have made immense impact in the area of healthcare by…

Q: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting…

A: The human genome is composed of sequences of nucleic acids, which are encoded as DNA(Deoxyribo…

Q: How are probes used to screen DNA libraries? Explain how a synthetic probe can be prepared when the…

A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…

Q: Describe the method of pronuclear injection to generate transgenic mice?

A: Transgenic mice are produced by putting DNA in their genome from another source. There are a number…

Q: which is the delivery techniques can bbe used to best transfer recombinant plasmids into E.coli…

A: ANSWER) The process of transferring the recombinant plasmids into the E.coli cells is called…

Q: Why are the recombinantDNA technology and thenucleus transplantationtechnology still dangerous?

A: Recombinant DNA technology basically refers to the joining of DNA molecules from two different…

Q: Explain gene-knockout technique.

A: According to the question, we have to provide an explanation of the gene knockout technique.  So,…

Q: Diagram the process by which restriction enzymes andDNA ligase are used to make recombinant…

A: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector.…

Q: The polymerase chain reaction can only be used to amplify genes that have been cloned and sequenced.…

A: The polymerase chain reaction was originally developed by the American biochemist Kary Mullis. The…

Q: What are transgenic Bacteria? Illustrate using any one example?

A: Genetic engineering is the modification of an organism’s genome for a beneficial outcome. Bacteria,…

Q: Why is the DNS method used in glucose assay?

A: 3,5-dinitrosalicylic acid (DNS) measure has been broadly utilized for the estimation of diminishing…

Q: when creating a transgenic animal Explain why you need to culture the E. coli bacteria on agar that…

A: Transgenic refers to the artificial introduction of one or even multiple DNA sequences from another…

Q: To understand the genes responsible for growth and infectivity in a disease-causing bacterial…

A: Site directed mutagenesis is technique in which Dna is altered and the altered gene product is used…

Q: Describe the benefits of using bacteria, yeast, mammalian and insect cells to make recombinant…

A: “Since you have asked multiple question, we will solve the first question for you. If youwant any…

Q: Explain the process of bacterial DNA extraction.

A: DNA extraction DNA extraction is a method of separation and isolation of pure DNA from other…

Q: What different techniques can be used for transcriptomeanalysis ?

A: The transcriptome is the set of all RNA transcripts including coding and non-coding in an individual…

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: How do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed…

A: In sickle cell anemia the blood cells become sickle shaped. This may result into painful blood flow,…

Q: Explain why recipient cells do not successfully takeup plasmids during natural transformation.

A: The alternation of genetic material of cell that results from direct uptake and incorporation of…

Q: Why is DNA microarray an important tool and explain How is DNA microarray used in detecting cancer?

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: Explain what restriction enzymes are, how they function and how they can be used to make recombinant…

A: Restriction enzymes are used to cut pieces of DNA.  Recombinant DNA is used in biotechnology to…

Q: Why are plasmids used as vector for DNA Recombination? What other vectors can  be used?

A: Plasmids are the extrachromosomal DNA that can replicate independently. They contain an origin of…

Q: What is the function of the Ti plasmid in the creation of transgenic organism? Explain your answer.

A: A plasmid can be defined as the extrachromosomal DNA of the bacterial which is small and separated…

Q: Explain The Discovery of CRISPR ?

A: The common technology   that can be used to edit genes is refers as the CRISPR. In other words you…

Q: True or false: Insulin is an example of a product that is generated using recombinant methods

A: Insulin is a peptide hormone produced by the beta cell of the pancreatic islets; it is considered to…

Q: Would it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?

A: Any group of Viruses that attach to the bacteria and infect the bacteria is known as bacteriophage.…

Q: What is the function of a restriction enzyme?

A: Restriction enzymes are enzymes that are isolated from bacteria that can cleave single-stranded or…

Q: If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type…

A: The incorporation of recombinant DNA into the bacterial cell is known as transformation. It leads to…

Q: Give any two examples of products, how transgenic animals can be used to produce biological…

A: Transgenic animals are the animals that have been produced by the use of biotechnology. It is the…

Q: In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells.…

A: Vector is a DNA sequence which serves as a vehicle for transferring the gene of interest from one…

Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?

A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…

Q: In the plaque assay, what is the precise origin of a single plaque?

A: Answer: Introduction: Virus is an intracellular infectious parasite consist of only one type of…

Q: Using a named example, discuss the advantages of using E. coli to produce recombinant proteins.

A: Recombinant DNA technology defines the artificial manipualtion of the genetic material to create the…

Q: According to the text, gene therapy currently includes all of the following methods EXCEPT:

A: A. replacing one sequence with another at the same locus via induced mutation.

Q: Explain the strategy by which a human gene can be cloned into a bacterial plasmid vector for…

A: Cloning vectors act as a vehicle to transfer desired foreign genetic material into a cell. All these…

Q: Describe with example, Why transgenic animals are produced?

A: Transgenic animals carry foreign genes. In them, the desired gene is introduced, using recombinant…

Q: Why is lambda DNA used as a marker?

A: Lambda deoxyribonucleic acid (DNA) is a linear, double-stranded phage DNA containing 12 bp…

Q: a bacteriophage has been isolated from the growth chambers that has evolved the capability to the…

A: In the modern age of technological revolution, various different techniques of genetic engineering…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.