Refer to the diagram below, which is representative of a single eukaryotic gene where the top strand is the template strand of DNA. Based on this diagram, which of the following statement is TRUE? 22 e 3' 5' None of the options are true. Both strands of nucleic acids depicted have the exact same nucleotide sequence. Both strands of nucleic acids depicted will consist of adenines, thymines, guanines, and cytosines. The N-terminus of the protein produced from this gene will correspond to the 5' end of the bottom strand. O The bottom strand corresponds to the DNA after splicing has taken place. 5' 3'

The following fragment of DNA is from the template strand. First determine the amino acids of the protein encoded by this sequence by using the table at the end of this document.  Then give the altered amino acid sequence of the proteins that will be found in each of the following mutations:   3’ – TAC AAG GCT CTA TTT GCC ACA ATC – 5’   The nucleotides are numbered 1-24 from left to right.   Mutant 1: A transition at nucleotide 9 Mutant 2: A transition at nucleotide 11 Mutant 3: A T to A transversion at nucleotide 15 Mutant 4: A one-nucleotide deletion at nucleotide 7 Mutant 5: A transition at nucleotide 13 Mutant 6: An addition of GGA after nucleotide 6

Given the following eukaryotic DNA strand, transcribe and translate the DNA into apolypeptide using the 3’ – 5’ strand as the template. You may use drawings, diagrams,colours and annotations to describe how the DNA strand will be synthesized into afunctional protein. (KEY: The letters SBMD are “made up” nucleic acids that depict non-coding regions in theDNA, hypothetically S pairs with B and M pairs with D).2.2. Describe what are missense mutations and its effects on structure and function usinghaemoglobin as an example (8).5’ - TATAAAAASSMSBMDATGSBDCCMBDBAATBSMDSTGTGTCCTMSBAG – 3’

Give the RNA molecule sequence transcribed from the following DNA sequence of a eukaryotic gene and with the correct 5' and 3' ends. DNA: 5'-ATAGGGCATGT-3' 3'-TATCCCGTACA-5'  <--- template strand Group of answer choices 5'-ATAGGGCATGT-3' 3'-UAUCCCGUACA-5' 5'-AUAGGGCAUGU-3' 3'-TATCCCGTACA-5'