Question: Referring To The Chromatogram, Answer The FollowingQuestions. Show Your Calculations. 1. What Is The Retention Time Of0.799он8.Ph0.699Ph„NH0.599HN7Ph-0.499PhPhPh0.399PhNH21Ph0.299PhPhPhPhPhPh 30.1990.099-0.0011.53.55.57.59.5Time (minutes)Referring to the chromatogram, answer the following questions. Show your calculations.1. What is the retention time of molecule 9?2. What happens to the peak area if sample amount is doubled?3. If run time is stopped at 5.5 mins, will molecule 8 & 6 be separated and quantified?4. If flow rate was doubled, will 8&6 be separated and quantified?5. Does a single peak at time T in chromatogram indicate only one compound is eluted?Absorbance unit

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Appl Of Ms Excel In Analytical Chemistry

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Chapter4: Least-squares And Calibration Methods

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Which statements are true for molecular exclusion chromatography?I Molecular exclusion chromatography separates on the basis of size.II Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume, and elute last.III The pores in the stationary phase are too small for large molecules to pass through, so large molecules elute first.IV Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography. A. I, II, III B. III and IV C. II only D. I and II only E. I, III, and IV
Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed:Rf Values: (A: 0.6, B: 0.9, C: 0.5, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method?2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the ?meaning of RT
In ion-exclusion chromatography, ions are separated from nonelectrolytes (uncharged molecules) by an ion-exchange column. Nonelectrolytes penetrate the stationary phase, whereas ions with charge of the same sign as that of the stationary phase are repelled by the stationary phase. Because electrolytes have access to less of the column volume, they are eluted before nonelectrolytes. A mixture of trichloroacetic acid (TCA, pKa 5 20.5), dichloroacetic acid (DCA, pKa 5 1.1), and monochloroacetic acid (MCA, pKa 5 2.86) was separated by passage through a cation-exchange resin eluted with 0.01 M HCl. The order of elution was TCA , DCA , MCA. Explain why the three acids are separated and the order of elution.

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Referring to the chromatogram, answer the following questions. Show your calculations. 1. What is the retention time of molecule 9? 2. What happens to the peak area if sample amount is doubled? 3. If run time is stopped at 5.5 mins, will molecule 8 & 6 be separated and quantified?