Research Center received a protein sample in a liquid form from her colleague recently. However, the colleague did not write down the concentration of the protein in the sample so that Dr. Kim has no idea how much proteins are in the sample. Fortunately, the colleague indicated the extinction coefficient at the maximum absorbance, which is e = 280,000 cm¯'M!. Thus, Dr. Kim measures the absorbance of the sample at ст different wavelengths with a spectrophotometer using a cuvette with a path length of 15 mm. The measurement result is shown below. What is the concentration of the sample? Plesae assume that there are no other compounds in the sample, except the proteins. 0.6 0.5 0.4 0.3 0.2 0.1 400 600 800 1000 1200 Wavelength (nm) Optical Absorbance
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
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