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RNA-Seq is a next-generation sequencing method used to quantitatively profile the cellular transcriptome. Researchers use RNA-Seq to compare the expression of genes under different environmental conditions or between different types of cells. Starting with isolated mRNA, there are three general steps in an RNA-Seq workflow: 1. Generate a cDNA library from cellular mRNA. 2. Add oligonucleotide adapters to the fragments of the cDNA library. 3. Use next-generation sequencing to identify transcriptionally active genes from the cDNA library. What is the role of the enzyme reverse transcriptase in an RNA-Seq workflow? Reverse transcriptase acts on cDNA bound to an adapter oligonucleotide during the sequencing process. Reverse transcriptase isolates RNA from other cellular components Reverse transcriptase catalyzes the addition of nucleotide adapters to fragments of the cDNA library. Reverse transcriptase produces cDNA fragments from RNA templates.

RNA-Seq is a next-generation sequencing method used to quantitatively profile the cellular transcriptome. Researchers use RNA-Seq to compare the expression of genes under different environmental conditions or between different types of cells. Starting with isolated mRNA, there are three general steps in an RNA-Seq workflow: 1. Generate a cDNA library from cellular mRNA. 2. Add oligonucleotide adapters to the fragments of the cDNA library. 3. Use next-generation sequencing to identify transcriptionally active genes from the cDNA library. What is the role of the enzyme reverse transcriptase in an RNA-Seq workflow? Reverse transcriptase acts on cDNA bound to an adapter oligonucleotide during the sequencing process. Reverse transcriptase isolates RNA from other cellular components Reverse transcriptase catalyzes the addition of nucleotide adapters to fragments of the cDNA library. Reverse transcriptase produces cDNA fragments from RNA templates.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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RNA-Seq is a next-generation sequencing method used to quantitatively profile the cellular transcriptome. Researchers use RNA-Seq to compare the expression of genes under different environmental conditions or between different types of cells. Starting with isolated mRNA, there are three general steps in an RNA-Seq workflow: 1. Generate a cDNA library from cellular mRNA. 2. Add oligonucleotide adapters to the fragments of the cDNA library. 3. Use next-generation sequencing to identify transcriptionally active genes from the cDNA library. What is the role of the enzyme reverse transcriptase in an RNA-Seq workflow? Reverse transcriptase acts on cDNA bound to an adapter oligonucleotide during the sequencing process. Reverse transcriptase isolates RNA from other cellular components. Reverse transcriptase catalyzes the addition of nucleotide adapters to fragments of the cDNA library. Reverse transcriptase produces cDNA fragments from RNA templates.
Transcribed Image Text:RNA-Seq is a next-generation sequencing method used to quantitatively profile the cellular transcriptome. Researchers use RNA-Seq to compare the expression of genes under different environmental conditions or between different types of cells. Starting with isolated mRNA, there are three general steps in an RNA-Seq workflow: 1. Generate a cDNA library from cellular mRNA. 2. Add oligonucleotide adapters to the fragments of the cDNA library. 3. Use next-generation sequencing to identify transcriptionally active genes from the cDNA library. What is the role of the enzyme reverse transcriptase in an RNA-Seq workflow? Reverse transcriptase acts on cDNA bound to an adapter oligonucleotide during the sequencing process. Reverse transcriptase isolates RNA from other cellular components. Reverse transcriptase catalyzes the addition of nucleotide adapters to fragments of the cDNA library. Reverse transcriptase produces cDNA fragments from RNA templates.
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