S. Why did you prepare a human serum plate?a. to see how closely related we are to ourselvesb. to serve as a control for the experimentc. to test for human DNAYes6. Was agglutination caused by an antigen-antibody reaction?NoSerumAntiserum7. Which substance contained the antibody?SerumAntiserum8. Which substance contained the antigen?HumanRabbit9. The antiserum was produced by a:

Our body is composed of blood that contains the plasma and the cellular part (RBCs, WBCs, and…

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3-2-1 Code It

6th Edition

ISBN:9781337660549

Author:GREEN

Publisher:GREEN

Chapter17: Cpt Pathology And Laboratory

Section: Chapter Questions

Problem 6MC

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a) What is “washing” in immunology? What types of interactions doeswashing interfere with? What molecules are used for effective“washing” in the immunology lab?b) Blocking is also a common feature of immunological assays. Why isblocking required?i. A student forgot to block their Western blot membrane beforeadding the secondary antibody. They continue and block themembrane after the secondary antibody step, assuming itdoesn’t matter which order the steps are completed in. Howwould this affect the results seen?ii. A student blocked their ELISA plate with 50 microlitres ofblocking buffer, although their coating, binding and detectionsteps were at 100 microlitres. How would this affect the resultsseen?
III. LAB REPORT You inherited your blood type from your parents. Your blood type is based on antigens found on your red blood cells named A or B and antibodies found in your plasma called a or b. See Table 1 for the antigen-antibody make-up of different blood types. Table 1 Blood type Antigens Antibodies A A b B B a AB A and B neither a nor b O neither A nor B both a and b A. When would clumping (or agglutination occur?
The immune system of a person is supressed. He was found positive for a pathogen in the diagnostic test ELISA.(a) Name the disease, the patient is suffering from.(b) Which pathogen is identified by ELISA test?(c) Which cells of the body are attacked by the pathogen?(d) Suggest preventive measure of the infection.
Describe 2 methods of immunotherapy currently in use, and the clinical results of one of these (ie, how often does it work well?)
How we can Treatment Severe Combined Immunodeficiency (SCID) by using gene therapy? Please answer at your own words,please..
Which technique allows the recovery of a protein from a sample such that the recovered protein can be used in subsequent experiments? a. monoclonal antibody cocktail b. enzyme-linked immunosorbent assay c. Western blot d. Southern blot e. immunoprecipitation I thought the answer is western blot but I got it wrong.. Would the answer be immunoprecipitation? I really have no idea please help..
Which DOES NOT describe monoclonal antibodies? Group of answer choices a. Monoclonal antibodies are produced in test tubes by hybridoma cells b. Monoclonal antibodies are specific to multiple antigens c. These antibodies are used in diagnostic tests d. The antibody producing cells may come from a non-human source
a. Explain why babies with agammaglobulinemia do not developopportunistic infections until about 6 months after birth.b. Explain why people with B-cell deficiencies can benefit fromartificial passive immunotherapy. Explain whether vaccinationwould work for them.c. Explain why T-cell deficiencies usually cause more severe effectsthan B-cell deficiencies.
What is the reason behind antibody diversity? Please answer in detail and fo not copy from anywhere please
40. Based on exposure to an atgen, which statement is accurate? a. the secondary response to the initial antigen is both faster and more intense than the primary response. b. the secondary repsonse to the intial antigen is both slower and more intense than the primary response. c. the primary repsonse to the intial innjection never subsides. d. the primary and secondary repsonses to the initial antigen are about equal.
Tuberculosis is a chronic respiratory condition caused by Mycobacterium tuberculosis, a high contagious bacterium. People with tuberculosis will often have a persistent (sometimes bloody) cough, fatigue, weight loss, and fever. M. tuberculosis produces a protein we will call “X”. A new test for tuberculosis checks for X antibodies in a person’s blood. If the antibodies are present, the result is “positive.” Why would X antibodies be present in someone’s blood? What specific kind of cells would produce them?
c) In performing an immunological procedure, you add 2 mL of stock serum to 8 mL of saline. Two 1/4 serial dilutions are then made. What is the dilution of serum contained in the final tube? d) How would you perform a 1/5 dilution of glucose stock solution in a final volume of 1 mL (using water as a diluent)? Write all the steps of the procedure and show all your calculations.

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