SCIENTIFIC INQUIRY • DRAW IT A researcher has developedan assay to measure the activity of an important enzymepresent in pancreatic cells growing in culture. She adds theenzyme’s substrate to a dish of cells and then measures theappearance of reaction products. The results are graphed as theamount of product on the y-axis versus time on the x-axis. Theresearcher notes four sections of the graph. For a short periodof time, no products appear (section A). Then (section B) thereaction rate is quite high (the slope of the line is steep). Next,the reaction gradually slows down (section C). Finally, thegraph line becomes flat (section D). Draw and label the graph,and propose a model to explain the molecular events occurringat each stage of this reaction profile.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
SCIENTIFIC INQUIRY • DRAW IT A researcher has developed
an assay to measure the activity of an important enzyme
present in pancreatic cells growing in culture. She adds the
enzyme’s substrate to a dish of cells and then measures the
appearance of reaction products. The results are graphed as the
amount of product on the y-axis versus time on the x-axis. The
researcher notes four sections of the graph. For a short period
of time, no products appear (section A). Then (section B) the
reaction rate is quite high (the slope of the line is steep). Next,
the reaction gradually slows down (section C). Finally, the
graph line becomes flat (section D). Draw and label the graph,
and propose a model to explain the molecular events occurring
at each stage of this reaction profile.
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