Please answer fast and both questions Shown below are single-stranded DNA probe and target sequences. Where on the target sequence will the probe anneal?Probe: 5'-CTAGT-3'b.d.Target: 5'-CTAGTGATCATGATCACTAG-3'a.C.O aO db.O Insufficient information to concludeO cThe following features or structures are more likely to be found in which molecule(s)?• bulges• internal loops• hairpins• non-Watson-Crick base-pairingDNARNANone of the molecules mentioned hereProteinsO RNA and DNA

In cells, DNA (Deoxyribonucleic acid) is the nucleic acid that functions as the original blueprint…

Shown below are single-stranded DNA probe and target sequences. Where on the target sequence will the probe anneal? Probe: 5'-CTAGT-3' b. d. Target: 5'-CTAGTGATCATGATCACTAG-3' a. C. O d. b. O Insufficient information to conclude The following features or structures are more likely to be found in which molecule(s)? • bulges • internal loops • hairpins • non-Watson-Crick base-pairing DNA RNA O None of the molecules mentioned here O Proteins O RNA and DNA

Shown below are single-stranded DNA probe and target sequences. Where on the target sequence will the probe anneal? Probe: 5'-CTAGT-3' b. d. Target: 5'-CTAGTGATCATGATCACTAG-3' a. C. O d. b. O Insufficient information to conclude The following features or structures are more likely to be found in which molecule(s)? • bulges • internal loops • hairpins • non-Watson-Crick base-pairing DNA RNA O None of the molecules mentioned here O Proteins O RNA and DNA

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
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How are DNA fragments separated on an agarose gel duning electrophoresis ? A. The electric current makes the fragments migrate through the geThe smaller fragments migrate through the gel the fastest. B. The heavier fragments fall faster than the lighter ones and therefore, reach the end of the gel first C. The fragments are sorted based on the shape of their folding The more complicated the folded shape is, the slower the travel rate is. D. The larger fragments have more charge and, therefore, move through the gel faster than the smaller fragments John Doe has two possible fathers. The DNA fragment sizes of John Doe, John Doe's mother, a possible fathers are as follows: John Doe's mother - 850 bp and 400 bp John Doe - 680 bp and 400 bp Possible father 1 - 680 bp and 500 bp Possible father 11 - 680 bp and 280 bp Which, If either of the two men could be John Doe’s father and why? A. both of the two men, because they and John Doe all have a 680 bp sized DNA fragment B.…
indicate which of the following statements is/are FALSE for qPCR A. The sequence order of steps for a qPCR is 1. Denature 2. Anneal 3. Detect 4. Extend B. qPCR estimates the quantity of template nucleic acid according to the change in fluorescence during the reaction. C. The estimation of nucleic acid quantity is performed at the end point after ~30 -35 PCR cycles D. The absorbance at 260 nm is used in qPCR to estimate the initial amount of DNA in the reaction. E. Intercalating dyes are added to the qPCR to estimate the quantity of dsDNA F. The fluorescent probes used in qPCR are extended by the polymerase
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With regard to dideoxy sequencing, which of the following statementsis false?a. The dideoxy nucleotides are fluorescently labeled.b. The dideoxy nucleotides cannot be incorporated into a growingDNA strand.c. When incorporated into a DNA strand, the dideoxynucleotidesprevent further growth of the strand.d. The dideoxy sequencing method is used to determine the basesequence of DNA.e. The dideoxy sequencing method requires the use of primers.
Please asap Original DNA template: 3'-ACGGTCAATTTGCTG-5 a) Transcribe the sequence. b) Translate the sequence. c) What type of mutation is present in the strand 3 '- ACGGTCAATATTGCTG - 5 d) Provide the entire mutated sequence of amino acids. e) Explain the effect that this mutation will have.
Which one of the following statements is true? a. Dideoxynucleotides signal the end of DNA replication in a cell b. None of the provided answers are true c. The DNA sequence determined by an autoradiogram should be identical to the template strand DNA sequence d. The probe hybridization solution used in the Sothern blot technique is approximately pH 10.0 e. The transfer buffer used in the Southern blot technique is approximately pH 7.0
The accompanying photo shows a sequencing gel from the original study that first sequenced the cystic fibrosis gene (J. R. Riordan et al. 1989. Science 245:1066–1073). From the photo, determine the sequence of the normal copy of the gene and the sequence of the mutated copy of the gene. Identify the location of the mutation that causes cystic fibrosis. (Hint: The CF mutation is a 3-bp deletion.)
Suppose the DNA synthesis that occurs in PCR is less accurate than cellular DNA synthesis (as in, PCR has a higher error rate than normal DNA synthesis). This difference in error would most likely be because the polymerase used in PCR: a) has a low level of 5' to 3' exonuclease activity b)has a low level of 3' to 5' exonuclease activity c) has a low temp at which it denatures d) adds to the 5' end of the growing DNA chain e) does not use template
the most efficient general strategy for whole genome sequencing is ? (a) double the coding sequence after sequencing the proteins (b) shotgun sequence and assemble based on overlaps (c) identify mutations that affect glycolysis (d) obtain recombinant DNA clone maps before starting the sequencing (e) obtain comprehensive SNP maps before determining the order of DNA clone
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