Staining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?

Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…

A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…

Q: Compare and contrast how dark-field, phase-contrast, differential interference contrast,…

A: The technique by which the smaller or minute particles are magnified for studying the features is…

Q: As with standard phase contrast microscopy,differential-interference contrast microscopy depends on…

A: Differential interference microscopy needs many optical parts, thus it may be terribly costly to…

Q: Differentiate between the limit of resolution of the typical light microscope and that of the…

A: The microscope is an instrument that helps us to visualize the objects that cannot be seen by our…

Q: Microscope You should always position the cells or tissues in the center of view before increasing…

A: One of the major principles of microscopy is to obtain a magnified image. This allows or permits the…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: Which of the following is never useful for observing living cells? O phase-contrast microscope…

A: Introduction All living organisms are made up of cells, which are the basic building components.…

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: If a microscope has a 40x objective lens in place and a 15x ocular lens, the total magnification of…

A: Microscope is used to see tiny objects in enlarged size to study the internal mechanism of object.…

Q: List and define the three properties of properly working microscope.

A: Answer: MICROSCOPE = It is an instrument which is used to see the microscopic organisms to see…

Q: During studying a thick heavily stained specimen under the microscope, it is better to light…

A: Microscopy is the technique of visualisation of microscopic specimens by using a microscope after…

Q: In the virtual microscope lab, the simulation made you add something to the slide before you could…

A: Microscopy is one of the important tools in microbiological analysis and understanding. The…

Q: discuss the microscopy technique that gives the best constrast image of non biological samples such…

A: Microscopy is an instrument used to magnify the size of the objects or particle which cannot be seen…

Q: What is the limit of resolution for a bright-field microscope?What defines this limit?

A: As a general  bright-field microscope, the  limit to magnification  is around 1300X and the limit of…

Q: What stain would you use to differentiate between bean and ant cells? Explain and include your…

A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…

Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…

A: Magnification and resolution are important because the magnification in larges the image and the…

Q: focusing

A: A light microscope is an apparatus or tool used in a biology laboratory to detect, magnify, and…

Q: Which of the following statements are true in the context of the microscope's field of view? I. It…

A: Microscope field of view (FOV) It is defined as the maximum area visible when looking through the…

Q: What limitation applies to most forms of electron microscopy?

A: When we use Electron microscopy then we face certain limitations or drawbacks. Here we will discuss…

Q: why is microtome important in microscopy?

A: Objects or organisms that cannot be viewed by the unaided eyes are studied under the microscope.…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes…

A: The compound microscope provides a 2-dimensional image of the specimen as there is just one path for…

Q: An object found with the 10x objective of the microscope may be viewed with the 40x objective…

A: A compound microscope has multiple lenses of different magnification.

Q: otal magnification of a microscope is determined by Multiple Choice dividing the objective…

A: The tiny and minute organisms that cannot be observed by unaided eyes are the microorganisms. These…

Q: Write in digital format please Describe the step-by-step process for taking micrographs on a…

A: Transmission electron microscopes use a beam of electrons to produce high-resolution images of a…

Q: A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X…

A: Introduction  Magnification refers to enlarging something's apparent size rather than its true size.…

Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?

A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…

Q: a. b. What is the basic principle of image formation using this microscopy technique? What can be…

A: There are few important points : Scanning electron microscopy : It is very powerful and versatile…

Q: Contrast the typical uses of bright-field, dark-field, and phase contrast microscopes.

A: Microscopy means the use of microscopes for studying the cells. Microscope is an indispensable…

Q: Light microscopy is used for which image? Image A Image B Image B, on the right O Image A, on the…

A: In the history of biological studies, various new tools and techniques have been developed so as to…

Q: Give the functions and magnifications of transmission electron microscope and scanning transmission…

A: Transmission electron microscope and scanning electron microscope are two types of electron…

Q: In negative staining, the acidic stain will penetrate the cell. In negative staining, it will create…

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: The above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective…

A: The eyepiece graticule is a scale that is placed in the eyepiece of a microscope containing scales…

Q: True or False: Ultrathin sections are used for light microscopy but are to thin to be stable under…

A: Microscopes are used to visualize objects that are not visible to the naked eye. In microbiology,…

Q: Staining of cells is often performed to enhance images acquired by brightfield microscopy.…

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Q: Describe the operating features of electron microscopes andhow they differ from optical microscopes…

A: Optical microscopes use light as a source for image formation. Unlike the light or optical…

Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…

A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…

Q: A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X…

A: Size of Object= 100 micron = 10 X 10-3 mm= 10-2 mm= 0.01 mm Size of Image= 10 mm Magnification= Size…

Q: Describe the use of fluorescent dyes in microscopy.

A: Microscopy is a technical field that involves utilizing microscopes to examine materials and things…

Q: A microscope that remains in focus regardless of whichobjective lens is rotated into place is…

A: The microscope is an almost widespread image of science, addressing our capacity to investigate the…

Q: Why is the resolution so superior in the electron microscope?

A: A beam of electrons that travel in wavelike patterns when accelerated to high speeds form the images…

Q: How is confocal scanning laser microscopy differentfrom fluorescence microscopy? In what ways are…

A: Microscope is an analytical instrument which is used to analyse, study and visualize internal…

Q: Why is cell imaging a preferred methos for studying cells

A: Cell imaging a preferred method for studying cells because it enables the visualization and…

Q: Why is methylene blue used to stain cells? What does it bind to?

A: Answer: Methyl blue is used to stain the cell because it is basic dye which can easily bind with…

Q: Transmission and scanning electron microscopy differ because transmission electron microscopy shows…

A: As per the guidelines, we are supposed to answer only one question. Kindly repost the other question…

Q: Explain briefly why the 100X objective lens of the light microscope is immersed in  oil.

A: The ability of a microscope to reveal an image that is numerous times larger in size than the…

Q: What is the principle of image formation on transmission election microscope and scanning electron…

A: The general biological microscope helps in the visualisation of the image of a particular sample…

Q: Discuss the microscopy technique that gives the best contrast image of non-biological samples such…

A: Scanning electron microscopy (SEM) SEM is considered as one of the most popular technique used for…

Q: How do stains used for light microscopy compare withthose used for electron microscopy?

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.