Study the diagram below. Put the number of the step in the diagram by its description in the list of steps at the bottom, which are not in the correct order. Then, answer the questions that follow. Human Cell 2 5 4 1 Sticky ends Bacterial Cell Bacterial chromosome 3 Recombinant DNA Plasmid Recombinant DNA is formed by mixing the donor gene with the plasmid Bacteria cell is screened & cloned (not shown) Plasmid put back into target (bacterial) cell Desired gene (for human insulin) is cut from donor DNA using restriction enzymes DNA is extracted from donor cell (human) Plasmid is removed from bacterial cell and cut open using restriction enzymes

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