Subtilisin is a protease that is often added to laundry detergents to help remove protein-based stains like blood and egg. To characterize subtilisin in the lab, the model substrate Ala-Ala-Pro-Phe-p- nitroanilide (AAPF-pNA) is often used. The KM for this substrate is 1.8 mM. At a substrate concentration of 400 μM, the observed vo is 9.6 µM s²¹. The enzyme concentration is 1 µM. Calculate the kcat for this enzyme. Provide your answer in units of s-¹. Kcat S-1

Subtilisin is a protease that is often added to laundry detergents to help remove protein-based stains like blood and egg. To characterize subtilisin in the lab, the model substrate Ala-Ala-Pro-Phe-p- nitroanilide (AAPF-pNA) is often used. The KM for this substrate is 1.8 mM. At a substrate concentration of 400 μM, the observed vo is 9.6 µM s²¹. The enzyme concentration is 1 µM. Calculate the kcat for this enzyme. Provide your answer in units of s-¹. Kcat S-1

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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