Supplementary Problems: Problem 1: At a magnification of 40x, a student measured 41 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 40x? Problem 2: At a total magnification of 100x, a student measures 16.4 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 100x? Problem 3: At a total magnification of 400x, a student measures 4.1 ocular micrometer divisions per millimeter. What is the distance, in micrometers, per ocular unit at 400x?
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- Please make comments on the clinical potential of Functional Near InfraredSpectroscopy (fNIRS)? Write down 3 issues and problems regarding fNIRSclinical applications?What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and dilution factors? What are 2 disadvantages?What is the diameter of field of view for the high-power objective in millimeters and micrometers?
- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What is the value at 1000x magnification? At 100x magnification?
- What information can be obtained from the slides prepared by the wet mount technique and the hanging mount technique? Give at least three (3) information. What are the limitations of each method (Provide two limitations)TRUE or FALSE only! 1. Problem: Calibrating ocular micrometer using the stage micrometer under the low power objective. Given: OD=27 SD=8 Each space on the stage micrometer=0.0001 inch Based on the given values, the ODV is equal to 0.753 2. Problem: Calibrating ocular micrometer using the stage micrometer under the low power objective. Given: OD=27 SD=8 Each space on the stage micrometer=0.0001 inch Based on the given values, the SDV is equal to 3.54 3. Inoculation chambers can be used in transferring bacterial culture while laminar flow cabinet can be used as permanent storage for biological samples.Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
- Practice problems: Do on folder paper. 1. You are observing a cell; total magnification 100X. It looks like about 7 cells will fit across the field of view's diameter. How large is this cell? Explain. 2. For the 2nd specimen, you are using a total magnification of 400X. It's harder to estimate this time, so you approximated that 12 cells would fit across the radius. Explain how you would find out the size of one cell.if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?[QUESTION EDIT REUPLOAD] What is the diameter of the field of view (DFV) of a 40x objective lens in the DFV of a 10x objective lens is 3mm? express your answer in micra Use the formula: M1 * DFV1= M2*DFV2