What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?

Q: Which of the following results in green output in spectrophotometry experiment? a. Violet light b.…

A: Spectrophotometry: it is used for quantitative analysis of spectra of various wavelengths to compare…

Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?

A: Spectrophotometry is the analytical technique by which the character of a substance is measured…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: What is the diameter of field of view for the high-power objective in millimeters and micrometers?

A: A microscope is a device that allows the examining of small objects, including cells. The picture of…

Q: How does a contaminant such as salt affect the absorption of light by a spectrophotometric sample?

A: Spectrophotometry is a method in which the absorption or transmission properties of a material is…

Q: What total magnification will be achieved if the 10× eyepiece and the 100× objective are…

A: The microscope is an instrument which used to view and observe tiny organism or an object that…

Q: What is the diameter of the scanning field of view in millimeters? ___________

A: The microscope is an instrument which used to view and observe tiny organism or an object that…

Q: WHAT ARE THE Steps in spectrophotometer

A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…

Q: What are the differences between scanner, low power objective, high power objective and oil…

A:

Q: Total Magnification is obtained by  Select one: A. Magnifying power of both the objective lens and…

A: Magnification is the term used for the increase in the size of the sample when seen under a…

Q: What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What…

A: Microscope is a instrument which is used to visualise the specimen that can't be seen by naked eyes…

Q: If a circular object or specimen extends halfway across the scanning field, what isits diameter in…

A: Field of view is defined as the area of the slide that is seen when an observer looks through a…

Q: what is the importance of getting rf value in chromotography tools?

A: Chromatography: Chromatography is an analytical technique in which compounds in a mixture are…

Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…

A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…

Q: What are the light sources used in UV-vis spectrophotometry.

A: In  case of visible spectrophotometers, the wavelengths measured are in the visible light range…

Q: Why is it necessary to use immersion oil when the 100X objective is used'

A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…

Q: What is the relationship between the working distance of an objective lens and its magnification…

A: Microscope is an optical instrument that uses a lens or an arrangement of lenses to magnify an…

Q: What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective…

A: The correct ans is 450X. The total magnification of specimen viewed with a 10x ocular lens and 45x…

Q: If the total magnification is 2000X with the use of a 10X ocular lens, what is the magnification of…

A: Answer- 200X

Q: what is the total magnification if the objective lens is 4x and the ocular lens is 10x?

A: Step 1 Microscopes are instruments that are used for observing small objects and finer internal…

Q: An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how…

A: Answer: MICROSCOPY : It is a technique for making small things to visible by magnifying through…

Q: What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x…

A: Given: Objective lens 1 magnification= 1000x DFV1 = ? Objective lens 2 magnification= 400x DFV2 =…

Q: the figure shows the result of the spectral analyzer for paper spectrophotometer without any…

A: Paper spectrophotometry is used in printing industry. In the printing industry, a spectrophotometer…

Q: What is the rule for determining total magnification? A. Ocular lens magnification + objective lens…

A: Microscopy is a technique of incorporating microscopes to enlarge the size of objects (invisible to…

Q: Here are the materials and method for the basic spectrophotometer experiment Materials: 1. Paper…

A: A spectrophotometer is a device used to measure the spectrum of visible light. The wavelength and…

Q: What is the total magnification possible with a 10x objective?

A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…

Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…

A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…

Q: How can you improve contrast when viewing a specimen?. What should you do if you have difficulty…

A: Step 1 Microscope (Greek. mikros− small, skipein− to see) is an instrument that is used for…

Q: Define each of the following terms:  A) What is resolution and how is resolution related to the…

A: According to the question, we have to provide the solution of the questions one by one. These…

Q: What is the main application of Atomic absorption spectrophotometer (AAS)?

A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…

Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…

A: A microscope is considered a laboratory instrument to observe microorganisms, small objects, cells,…

Q: What is the total magnification of 4x objective lens and diameter of field of view in micrometers?

A: Magnification: The amplifying power or we can say, degree to which the object is seen or viewed…

Q: What solution goes into the cuvette when you’re zeroing the spectrophotometer?

A: Spectrophotometer is a device that is used to calculate the absorbance of the light passing through…

Q: If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying…

A: Magnification is the process of enlarging the apparent size. This enlargement is quantified by a…

Q: Do the calibration factor computed varies according to different magnification (objective lenses…

A: By simply accounting for the inaccuracy, the Correction Factor allows us to attain more accurate…

Q: spectrophotometry, what is the role of the green background in the set-up? What does it represent in…

A: Introduction:- Spectrophotometry is a technique for determining how much light a chemical substance…

Q: what objective lens is the oil objective lens?

A: We have to determine the objective lens that is used for oil immersion.

Q: If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x,…

A: According to the question, the total magnification of a microscope is 700X.…

Q: is the reason for using blind tube in spectrophotometric and calorimetric studie

A: A method which is used to  measure how much a chemical substance absorbs light by measuring the…

Q: What information can be obtained from the slides prepared by the wet mount technique and the hanging…

A: Introduction :  The wet mount and hanging mount techniques are used to observe living…

Q: Question 5 What is the total magnification of a sample with an ocular lens power of 15X and using a…

A: A compound microscope has two lens one is ocular or eyepiece lens and objective lens. Objective lens…

Q: What is the differences between ocular magnification and objective magnification?

A: Introduction  A microscope's objective lens is the one near the sample at the bottom. It's an…

Q: What are the significance of UV/Vis spectrophotometer in the experiment complexation.

A: UV/VIS spectrophotometer is the technique used to determine the optical properties  such as…

Q: How do you know the range in microliters within which a given micropipette can accurately measure?

A: A micropipette is a device used to transfer small volume of samples . It is a vital part in…

Q: A michroscope has a 20x ocular and two objectives of 10x and 43x respectively: calculate the…

A: Apparent magnification of the objective is provided by the proportion of the viewing angle of its…

Q: What is the stationary and mobile phase of spectrophotometry?

A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…

Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?

A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…

Q: If the specimen measures 150mm and you draw it 50mm, how many times you have it reduced?

A: Magnification is defined as the ability to make smaller object to see it in larger manner.  In this…

Q: what is experimental error that may occur when reading spectrophotmeter

A: Spectrophotometry is a technique for determining how much light a chemical substance receives by…