Suppose I label the newly synthesized DNA with P, grind up the treated cells and run their single stranded DNA on a gel. There is a radioactive band corresponding to very large (>10kb) DNA fragments and a band corresponding to ~200bp fragments. What are the proportions of each by mass? A. >99% very large. B. ¾ very large, ¼ small. C. 2/3 very large, ¼ small. D. ½ very large, ½ small. E. >99% small.

Suppose I label the newly synthesized DNA with P, grind up the treated cells and run their single stranded DNA on a gel. There is a radioactive band corresponding to very large (>10kb) DNA fragments and a band corresponding to ~200bp fragments. What are the proportions of each by mass? A. >99% very large. B. ¾ very large, ¼ small. C. 2/3 very large, ¼ small. D. ½ very large, ½ small. E. >99% small.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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Similar questions

In a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the DNA structure. What is the fundamental physical basis for the absorbance increase? a. the effective concentration of DNA changes b. the absorbance of DNA is highly sensitive to temperature c. native DNA absorbs light more strongly than denatured DNA because the bases are stacked d. denatured DNA absorbs light more strongly than native DNA because the bases have become unstacked
C. What is the purpose of the ethidum bromide that you add to the gel? Why do you need to be careful handling it? What type of light do you view the gel under if you use EtBr? D. What is the running buffer called that you submerge your gel in? E. What is the purpose of the molecular weight ladder? F. What are the 2 purposes of the DNA loading buffer?
The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your answers, show how you came up to each result?(a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in micrometer?
If you have G-C rich DNA versus A-T rich DNA a.Will the melting temperatures be the same/ Why? b.Will the annealing temperature be the same? c. Which of these categories is prone to have more errors?
In Noll’s experiment to test the beads-on-a-string model, exposure of nuclei to a low concentration of DNase I resulted ina. a single band of DNA with a size of approximately 200 bp.b. several bands of DNA in multiples of 200 bp.c. a single band of DNA with a size of 100 bp.d. several bands of DNA in multiples of 100 bp
If you have G-C rich DNA versus A-T rich DNA. (a)Will the melting temperature be the same? Why? (b) Will the annealing temperature be the same? (c) Which of the categories in (a) above is more prone to errors?
In experiments using polymerase chain reactions (PCR), it is often more difficult to amplifythrough regions of DNA that are high in GC content versus those regions that are either lower inGC content or are AT-rich. Based on your knowledge of DNA structure, explain why.
Enzymes that break down DNA catalyze the hydrolysis of the covalent bonds that join nucleotides together. What would happen to DNA molecules treated with these enzymes? Group of answer choices A. All bases would be separated from the deoxyribose sugars B. The purines would be separated from the deoxyribose sugars C. The phosphodiester linkages between deoxyribose sugars would be broken D. The two strands of the double helix would separate E. The pyrimidines would be separated from the deoxyribose sugars
Which of the following does not contribute to the stability of the DNA? A. The presence of hydrogen between nitrogenous bases. B. Presence of the N-glycosidic bond between the nitrogenous base and phosphate group C. Presence of phosphodiester bond between the sugar and phosphate group on the sugar-phosphate backbone. D. Hydrophobic interaction between stacked nitrogenous bases.
Visually compare the intensity of your team’s Plant-DNA sample with the intensity of the 3 kb band on the DNA ladder (see figure posted below). For example, if your Plant- DNA sample closely matches the 3 Kb band, this represents approximately 125 ng of total DNA. Divide the approximate mass (ng) of DNA in your sample by the volume loaded in the gel minus the loading dye added (i.e., 12 – 2 = 10 μL, since you loaded 12 μL from a 24 μL sample that contained 20 μL DNA prep + 4 μL loading dye. What is the DNA concentration of your sample? ______ng/μL
When a double-stranded DNA molecule is exposed tohigh temperature, the two strands separate, and themolecule loses its helical form. We say the DNA hasbeen denatured. (Denaturation also occurs whenDNA is exposed to acid or alkaline solutions.)a. Regions of the DNA that contain many A–T basepairs are the first to become denatured as the temperature of a DNA solution is raised. Thinkingabout the chemical structure of the DNA molecule, why do you think the A–T-rich regionsdenature first?b. If the temperature is lowered, the original DNAstrands can reanneal, or renature. In addition to thefull double-stranded molecules, some molecules ofthe type shown here are seen when the moleculesare examined under the electron microscope. Howcan you explain these structures?
You perform an experiment to determine the complete melting profile of a relatively long DNA duplex with 80% G:C base pairs. Sketch a graph of Absorbance at 260 nm (Y-axis) vs. Temperature (X-axis). Label the location of the Tm and describe what is happening to the DNA structure in each segment of the graph (lower Temps, highest Temps, at Tm).