Table 2. Effect of injection volume and temperature on retention time, peak width and resolution. Peak Width isopropanol (min) O544 0.0531 1.06I5 0.0647 0.0649 Peak Width Resolution RT MeOH (min) isopropanol (min) 1904 1.727 1.696 1712 1.741 Oven RT MEOH Temp (°C) (min) 1.5670.0720 0.0385 0.0319 O.0227 60 80 1-592 100 1.648 120 1.683 140 7. Comment on the relationship between oven temperature and resolution for the 10 uL injection. Is there any temperature at which the resolution is no longer sufficient to give baseline separation? 8. Comment on and explain any differences that are observed in the chromatogram and temperature dependence of the resolution between the 1 ul and 10 ul injection volumes.
Table 2. Effect of injection volume and temperature on retention time, peak width and resolution. Peak Width isopropanol (min) O544 0.0531 1.06I5 0.0647 0.0649 Peak Width Resolution RT MeOH (min) isopropanol (min) 1904 1.727 1.696 1712 1.741 Oven RT MEOH Temp (°C) (min) 1.5670.0720 0.0385 0.0319 O.0227 60 80 1-592 100 1.648 120 1.683 140 7. Comment on the relationship between oven temperature and resolution for the 10 uL injection. Is there any temperature at which the resolution is no longer sufficient to give baseline separation? 8. Comment on and explain any differences that are observed in the chromatogram and temperature dependence of the resolution between the 1 ul and 10 ul injection volumes.
Chapter34: Miscellaneous Separation Methods
Section: Chapter Questions
Problem 34.9QAP
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2
Transcribed Image Text:9. Is it better to use a small injection volume or a large injection volume. In your
answer, explain what happens in the GC instrument that makes one better than the
other?
Transcribed Image Text:Table 2. Effect of injection volume and temperature on retention time, peak
width and resolution.
Peak Width
isopropanol
(min)
O544
0.0531
O.0615
0.0647
0.0649
Oven
RT MEOH
Peak Width
RT
Resolution
MeOH (min) isopropanol
(min)
1904
1.727
1.696
1.712
1.741
Temp (°C) (min)
60
80
100
120
140
1.5670.0720
0.0385
0.0319
0.0227
-592
1-648
1.683
7. Comment on the relationship between oven temperature and resolution for the 10
uL injection. Is there any temperature at which the resolution is no longer sufficient to
give baseline separation?
8. Comment on and explain any differences that are observed in the chromatogram and
temperature dependence of the resolution between the 1 uL and 10 ul injection
volumes.
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