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Task 2: Find where the primers bind to in the gene sequences on Moodle (kan has been provided for you as a reference) Gene Forward Primer 5' to 3' lacz fliE Size of gene (bp) 3075 315 rpoA 990 nagC 1221 GCTCAGAACGTACCACCG ompA 1041 GCCGGGATACCTTTGG lexA 609 GTTCGACTTTATTGCCCTG kan cassette 816 CCTGGTATCGGTCTGC GGAAGCCCGATGCGCCAGAG 549 tet casette 1148 CTATAAACCGCACTGTGA GTCCTGGATTCCTCAGGT The reverse primer is the reverse compliment - remember to reverse compliment when looking for it in the sequences. Kan has been completed for you GTTAACCGTCACGAGCATC ACAGGGGATTGAAGGGGTTA Reverse primer 5' to 3' CGTTCAGGAAGATATCC CGCTGCCAACGCTGC GGCCCGACCCATGAAAAC GGCACGCAAAGGCGTTATTGA Expected size of PCR product (bp) TGATCACACTCGGGTGATTAC CGCACCTGAATCCCCATTTGC GGATCGATTGTGCCGTTGGTTT

Task 2: Find where the primers bind to in the gene sequences on Moodle (kan has been provided for you as a reference) Gene Forward Primer 5' to 3' lacz fliE Size of gene (bp) 3075 315 rpoA 990 nagC 1221 GCTCAGAACGTACCACCG ompA 1041 GCCGGGATACCTTTGG lexA 609 GTTCGACTTTATTGCCCTG kan cassette 816 CCTGGTATCGGTCTGC GGAAGCCCGATGCGCCAGAG 549 tet casette 1148 CTATAAACCGCACTGTGA GTCCTGGATTCCTCAGGT The reverse primer is the reverse compliment - remember to reverse compliment when looking for it in the sequences. Kan has been completed for you GTTAACCGTCACGAGCATC ACAGGGGATTGAAGGGGTTA Reverse primer 5' to 3' CGTTCAGGAAGATATCC CGCTGCCAACGCTGC GGCCCGACCCATGAAAAC GGCACGCAAAGGCGTTATTGA Expected size of PCR product (bp) TGATCACACTCGGGTGATTAC CGCACCTGAATCCCCATTTGC GGATCGATTGTGCCGTTGGTTT

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Task 2: Find where the primers bind to in the gene sequences on Moodle (kan has been provided for you as a reference) Gene Size of gene (bp) 3075 315 Грод 990 nagC 1221 ompA 1041 lexA 609 kan cassette 816 tet casette 1148 lacz fliE Forward Primer 5' to 3' GTTAACCGTCACGAGCATC ACAGGGGATTGAAGGGGTTA CGTTCAGGAAGATATCC CGCTGCCAACGCTGC GGCCCGACCCATGAAAAC GGCACGCAAAGGCGTTATTGA Reverse primer 5' to 3' TGATCACACTCGGGTGATTAC CGCACCTGAATCCCCATTTGC GGATCGATTGTGCCGTTGGTTT Expected size of PCR product (bp) GCTCAGAACGTACCACCG GCCGGGATACCTTTGG GTTCGACTTTATTGCCCTG CCTGGTATCGGTCTGC GGAAGCCCGATGCGCCAGAG 549 CTATAAACCGCACTGTGA GTCCTGGATTCCTCAGGT The reverse primer is the reverse compliment - remember to reverse compliment when looking for it in the sequences. Kan has been completed for you
Transcribed Image Text:Task 2: Find where the primers bind to in the gene sequences on Moodle (kan has been provided for you as a reference) Gene Size of gene (bp) 3075 315 Грод 990 nagC 1221 ompA 1041 lexA 609 kan cassette 816 tet casette 1148 lacz fliE Forward Primer 5' to 3' GTTAACCGTCACGAGCATC ACAGGGGATTGAAGGGGTTA CGTTCAGGAAGATATCC CGCTGCCAACGCTGC GGCCCGACCCATGAAAAC GGCACGCAAAGGCGTTATTGA Reverse primer 5' to 3' TGATCACACTCGGGTGATTAC CGCACCTGAATCCCCATTTGC GGATCGATTGTGCCGTTGGTTT Expected size of PCR product (bp) GCTCAGAACGTACCACCG GCCGGGATACCTTTGG GTTCGACTTTATTGCCCTG CCTGGTATCGGTCTGC GGAAGCCCGATGCGCCAGAG 549 CTATAAACCGCACTGTGA GTCCTGGATTCCTCAGGT The reverse primer is the reverse compliment - remember to reverse compliment when looking for it in the sequences. Kan has been completed for you
nagC primers M L1 L2 L3 L4 ||||||||||||| lacZ primers M L1 L2 L3 L4 fliE primers M L1 L2 L3 L4 I ompA primers M L1 L2 L3 L4 ||||||||||||| tet primers M L1 L2 L3 L4 kan primers M L1 L2 L3 L4 kan primers M L1 L2 L3 L4 kan primers M L1 L2 L3 L4 bp -10,000 -8,000 -6,000 -5,000 -4,000 -3,000 -2,500 -2,000 -1,500 -1,000 -750 -500 -250, 253 1.2% agarose k I=
Transcribed Image Text:nagC primers M L1 L2 L3 L4 ||||||||||||| lacZ primers M L1 L2 L3 L4 fliE primers M L1 L2 L3 L4 I ompA primers M L1 L2 L3 L4 ||||||||||||| tet primers M L1 L2 L3 L4 kan primers M L1 L2 L3 L4 kan primers M L1 L2 L3 L4 kan primers M L1 L2 L3 L4 bp -10,000 -8,000 -6,000 -5,000 -4,000 -3,000 -2,500 -2,000 -1,500 -1,000 -750 -500 -250, 253 1.2% agarose k I=
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