Test Reagents Used Positive Color Purpose Structure of Chemical Change 1. Biuret test 1% CuSO4 in Aqua blue to General test for 1%NAOH purple deep color proteins NH R- Cu -R HN- 2. Ninhydrin test 3. Xanthoproteic cest 4. Millons test
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- Benedict's test Green precipitate A. reducing sugars not presnt B. Reducing sugar presennt Seliwanoff's Test Colorless solution A. Aldose present B. Ketose present C. Neither aldose or ketose present Iodine Test Colorless to brown solution A. Amlyose present B. Amlyose not present1. Identify what test is being described: Refers to the breaking of peptide bonds that connect amino acids to compose protein ? A. Hydrolysis B. Denaturation C. Heat denaturation D. Organic solvent denaturation E. Biuret test F. Hopkins – Cole Reaction G. Millon’s test H. Ninhydrin Test I. Sulfur test J. Xanthroproteic Test K. Chromatography L. Paper chromatography M. Competitive inhibition N. Noncompetitive inhibition O. Rancidity P. Hydrogenation 2. Identify what test is being described: Test that detects the free amino group in amino acids ? A. Hydrolysis B. Denaturation C. Heat denaturation D. Organic solvent denaturation E. Biuret test F. Hopkins – Cole Reaction G. Millon’s test H. Ninhydrin Test I. Sulfur test J. Xanthroproteic Test K. Chromatography L. Paper chromatography M. Competitive inhibition N. Noncompetitive inhibition O. Rancidity P. Hydrogenation 3. Identify what test is being described: A foreign substance, which is structurally similar to the substrate, competes for…IIC. ZAK'S TEST The figure below summarizes the results for acrolein test. Provide the correct inference for the given result.PUT A CHECK Positive for cholesterol Negative for cholesterol A B C
- Bial’s Orcinol Test Reagent’s Composition Observations: Test Sample Observations Water Glucose Fructose Arabinose Inferences: What is the principle behind this test? What kind of sugars will give a positive result to this test?1. The adsorption solution used in SO2 analysis is…a. Tetrachloromercurate(V)b. Tetrachloromercurate(I)c. Tetrachloromercurate(III)d. Tetrachloromercurate(II) 2. Storage of samples under special conditions is carried out with the aim of…a. Samples that do not enter airb. Avoid repeated samplingc. So that it doesn't rotd. Avoid chemical reactionsMultiple choice (Refer to picture) Which of the statements is NOT TRUE about the sample in Salkowski test? * Lower layer becomes red with a green fluorescence A molecule of cholesterol reacts with sulfuric acid to yield bi-cholestadien There should be an excess sulfuric acid to convert bi-cholestadien to bisulfonic acid of bi-cholestadien. It is best to shake the solution for a more accurate result.
- 12. Identify what test is being described: Test for the presence of indole group ? A. HydrolysisB. DenaturationC. Heat denaturationD. Organic solvent denaturationE. Biuret testF. Hopkins – Cole ReactionG. Millon’s testH. Ninhydrin TestI. Sulfur testJ. Xanthroproteic TestK. ChromatographyL. Paper chromatographyM. Competitive inhibitionN. Noncompetitive inhibitionO. RancidityP. Hydrogenation 13. Identify what test is being described: Process of adding hydrogen to unsaturated fatty acids ? A. HydrolysisB. DenaturationC. Heat denaturationD. Organic solvent denaturationE. Biuret testF. Hopkins – Cole ReactionG. Millon’s testH. Ninhydrin TestI. Sulfur testJ. Xanthroproteic TestK. ChromatographyL. Paper chromatographyM. Competitive inhibitionN. Noncompetitive inhibitionO. RancidityP. Hydrogenation 14. Identify what test is being described: Yields blue color solution for proteins containing fewer than two peptide bonds. ? A. HydrolysisB. DenaturationC. Heat denaturationD. Organic solvent…Polysaccharides generally do not give a positive test with Fehling’s solution. Why? *Sucrose + H2O(negative control): Colorless to brown solution: Blue solution Conclusion for Iodine test A. amylose present B. amylose not present Conclusion for Benedict's test A. reducing sugars not present B. reducing sugars present Sucrose + HCl(potential hydrolysis): unchanged compared to sucrose+water: Conclusion for Iodine test A. more amylose present B. LESS amylose present C. NO change Conclusion for Benedict's test a. more reducing sugar present b. less reducing sugar present c. no change Did hydrolysis of sucrose occur in the presence of acid? A. Yes B. no
- Explain main difference would you see when conducting the nitrous acid test with aniline vs. amino-cyclohexane? Be specific.You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.1. Prepare 200 mL of an isotonic aqueous solution of thimerosal (MW 404.84; L iso 3.4). The concentration of this antiinfective drug is 1:5000. a. Calculate the E value. b. Adjust the Isotonicity of the solution using NaCl.