TESTS FOR GRAM POSITIVE ORGANISMS PART II Experiment 8C lab period 2 We will continue to introduce new tests used for gram-positive organism. Be sure to review results for BAP, MSA, and Coagulase tests from last lab period Antibiotic Susceptibility Tests Two separate tests for antibiotic susceptibility will performed. These tests are used to differentiate between alpha and beta hemolytic organisms. Alpha hemolytic organisms (Streptococcus pneumoniae and Streptococcus mitis) are tested for their susceptibility to Taxos P (optochin). The beta hemolytic organisms (Streptococcus pyogenes and Streptococcus agalactiae) are tested with Taxos A (bacitracin). A zone of inhibition of growth around the Taxos disc indicates that the organism is susceptible/sensitive to that antibiotic. Taxos A (bacitracin sensitivity testing) This is a differential test used to distinguish between organisms sensitive to the antibiotic bacitracin and those not. Bacitracin is a peptide antibiotic produced by Bacillus subtilis. It inhibits cell wall synthesis and disrupts the cell membrane. This test is commonly used to distinguish between the b-hemolytic streptococci: Streptococcus agalactiae (bacitracin resistant) and Streptococcus pyogenes (bacitracin sensitive). The plate below was streaked with Streptococcus pyogenes; notice the large zone of inhibition surrounding the disk. Taxos P (optochin sensitivity testing) This is a differential test used to distinguish between organisms sensitive to the antibiotic optochin and those not. This test is used to distinguish Streptococcus pneumoniae (optochin sensitive) from other a- hemolytic streptococci (optochin resistant). Procedure:
TESTS FOR GRAM POSITIVE ORGANISMS PART II Experiment 8C lab period 2 We will continue to introduce new tests used for gram-positive organism. Be sure to review results for BAP, MSA, and Coagulase tests from last lab period Antibiotic Susceptibility Tests Two separate tests for antibiotic susceptibility will performed. These tests are used to differentiate between alpha and beta hemolytic organisms. Alpha hemolytic organisms (Streptococcus pneumoniae and Streptococcus mitis) are tested for their susceptibility to Taxos P (optochin). The beta hemolytic organisms (Streptococcus pyogenes and Streptococcus agalactiae) are tested with Taxos A (bacitracin). A zone of inhibition of growth around the Taxos disc indicates that the organism is susceptible/sensitive to that antibiotic. Taxos A (bacitracin sensitivity testing) This is a differential test used to distinguish between organisms sensitive to the antibiotic bacitracin and those not. Bacitracin is a peptide antibiotic produced by Bacillus subtilis. It inhibits cell wall synthesis and disrupts the cell membrane. This test is commonly used to distinguish between the b-hemolytic streptococci: Streptococcus agalactiae (bacitracin resistant) and Streptococcus pyogenes (bacitracin sensitive). The plate below was streaked with Streptococcus pyogenes; notice the large zone of inhibition surrounding the disk. Taxos P (optochin sensitivity testing) This is a differential test used to distinguish between organisms sensitive to the antibiotic optochin and those not. This test is used to distinguish Streptococcus pneumoniae (optochin sensitive) from other a- hemolytic streptococci (optochin resistant). Procedure:
Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Margaret Rodriguez, Paul Price
Chapter6: Microbial Viability And Growth
Section: Chapter Questions
Problem 4UTM
Related questions
Question
This expiremwnt was carried out .
In no less than 30 words what can uconcluded from this lab
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Microbiology for Surgical Technologists (MindTap …
Biology
ISBN:
9781111306663
Author:
Margaret Rodriguez, Paul Price
Publisher:
Cengage Learning
Microbiology for Surgical Technologists (MindTap …
Biology
ISBN:
9781111306663
Author:
Margaret Rodriguez, Paul Price
Publisher:
Cengage Learning