An otic product was subjected to AET and tested with the challenge microorganisms. An initial concentration of 2 x 10^5 cfu/mL of Escherichia coli was computed. Compute for the log reduction from the initial calculated count if the cfu/mL obtained is 1.2 x 10^4 at 7 days.
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An otic product was subjected to AET and tested with the challenge microorganisms. An initial concentration of 2 x 10^5 cfu/mL of Escherichia coli was computed. Compute for the log reduction from the initial calculated count if the cfu/mL obtained is 1.2 x 10^4 at 7 days.
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- Consider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flowGiven the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.
- If the inoculated lactose broth cube exhibits acid production but without gas formation, will you consider this as a negative or positive result? Explain Why is the MPN test qualitative rather than quantitative? Why is the MPN test qualitative rather than quantitative? Why is MPN test qualitative rather than quantitativeFor each of the following aspects, compare and contrast the EnteroPluri-Test System to the API® 20E System for Enterobacteriaceae identification. a. time requirement b. specimen preparation c. tests utilized d. anaerobic conditions e. interpretation of resultsIf a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol. true or false
- Thermotolerant coliforms are often referred to as "faecal coliforms", although the organisms may not be of faecal origin. The time interval between collection of the sample and testing shoud not exceed 6 hours, why is this?After a series of experiments, bacterial cultures were identified for disposal. How can these be properly decontaminated prior to disposal according to biosafety guidelines? METHOD:RATIONALE:A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.