The 1000 aa protein (Protein X) you are studying has several putative caspase cleavage sites. You don't know how many of them are actually cleaved during apoptosis. Two of them are close enough together that it would be difficult to differentiate the sites just based on the size of the cleavage products. You decide to do some site- directed mutagenesis to figure out what is cleaved and generate mutant constructs for each potential site. You express the proteins in cell culture, induce apoptosis, and do the following Western blot. D380E D835E D840E D835E = D380E/ D380E/ D835E/ D840E D840E WT First: What antibody did you use here for the Western blot? anti-Apoptosome

The 1000 aa protein (Protein X) you are studying has several putative caspase cleavage sites. You don't know how many of them are actually cleaved during apoptosis. Two of them are close enough together that it would be difficult to differentiate the sites just based on the size of the cleavage products. You decide to do some site- directed mutagenesis to figure out what is cleaved and generate mutant constructs for each potential site. You express the proteins in cell culture, induce apoptosis, and do the following Western blot. D380E D835E D840E D835E = D380E/ D380E/ D835E/ D840E D840E WT First: What antibody did you use here for the Western blot? anti-Apoptosome

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter7: Development And Sex Determination

Section: Chapter Questions

Problem 19QP: Equalizing the Expression of X Chromosome Genes in Males and Females Individuals with an XXY...

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Concept explainers

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.

Question

Anti apoptosome

anti N

anti protein X

anti caspase

based on thé data above where is the WT protein actually cleaved

D380

D380 and D835

D835

D835 and D840

D840

all are cleaved

none are cleaved

D380 and D840

if you compared the size of WT and the D380E mutant before apoptosis on a western blot how would they appear?

D380E would be larger

they would be indistinguishable

WT would be larger

how many amino acids long is the larger band in the lane labeled D380E/D835E

The 1000 aa protein (Protein X) you are studying has several putative caspase
cleavage sites. You don't know how many of them are actually cleaved during
apoptosis.
Two of them are close enough together that it would be difficult to differentiate the
sites just based on the size of the cleavage products. You decide to do some site-
directed mutagenesis to figure out what is cleaved and generate mutant constructs
for each potential site. You express the proteins in cell culture, induce apoptosis, and
do the following Western blot.
D380E/ D380E/ D835E/
D380E D835E D840E D835E D840E D840E WT
11
First: What antibody did you use here for the Western blot?
O anti-Apoptosome
#tv A
MacBook Air
X

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