The Ames Test... a. is always used to detect cancer-causing virus b. has the potential to completely replace the need of animal testing of potential carcinogens c. uses a virus constructed to be susceptible to mutagenesis caused by mutagens d. includes liver extract in the assay to determine the mutagenicity of the metabolites of test samples e. is positive for all human carcinogens

The Ames Test... a. is always used to detect cancer-causing virus b. has the potential to completely replace the need of animal testing of potential carcinogens c. uses a virus constructed to be susceptible to mutagenesis caused by mutagens d. includes liver extract in the assay to determine the mutagenicity of the metabolites of test samples e. is positive for all human carcinogens

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter15: Biotechnology

Section: Chapter Questions

Problem 8SA: PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genome

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(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.
Pro's/con's of genetic testing. This can be as simple as for birth defects or in more complicated risk for different types of cancer.
Describe how Ames Test works to identify carcinogenic using back mutations (reversible)
A company that manufactures cleansing products has synthesized two new organic compounds , chemicals A and B, which improve the cleaning power of dishwashing detergents. To determine the mutagenic capacity of the chemicals , the company tested the effect of the chemicals on Salmonella cells requiring histidine to grow ( His- cells ). The table below shows the results of the test . A. Name the test used by the company . B. What does the test detect or estimates ? C. Which chemical (s) would you identify as containing a mutagen ? Why ? D. What is the purpose of test 1 ? Why are colonies detected in test 1? E. Which chemical ( s) would you identify as possible antimutagen ? Why ?
Explain in detail the AMES test which is used to test for the mutagenic potential of different compounds. Include an explanation of the AMES tester strains and how they work, why the liver is essential to this test and specifically why the control plate is necessary.
PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genome
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Explain with help of diagrams the mutagenic effects on DNA of following mutagens? Below is an example i. Ultraviolet light ii. X- raysiii. Base analogsiv. Hydroxylaminev. nitrous acidvi. ethylmethane sulfonate