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And to follow up previous question, how do you exactly identify the mutations in driving gene, as I need to give evidence.
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- explain the following terms: genomics, mutations(variants) & proteomicsThe accompanying photo shows a sequencing gel from the original study that first sequenced the cystic fibrosis gene (J. R. Riordan et al. 1989. Science 245:1066–1073). From the photo, determine the sequence of the normal copy of the gene and the sequence of the mutated copy of the gene. Identify the location of the mutation that causes cystic fibrosis. (Hint: The CF mutation is a 3-bp deletion.)Briefly answer the question: Exome sequencing to identify a mutation that could cause a particular set of symptoms in a patient can reveal another genetic condition that has not yet been detected. Under what circumstances, if any, do you think patients should receive such "secondary findings"?
- What detection method could be used to determine the mutagen added to a microbial culture resulting in an altered gene A of the organism and what results could be achieved from that?I have complete the table that required. Indicate which mutations were success why some were not from the size about. indicate at what stage any of the mutagenesis failed.Explain the Detection of the sickle-cell mutation by sequencing of PCR products?
- You want to identify and compare the gene expression profile of several genes in a disease. Will you be able achieve this using Q-PCR technique? Can you identify a potential mutation (the nucleotide change) within a gene sequence using conventional PCR? If not, then what approach you should take?Explain how the fluctuation test and replica plating haveshown that mutations arise randomly andspontaneouslyExplain how site directed mutagenesis can be accomplished using M13 bacteriophage. Using this method often results in only 5% of plaques with mutant gene. Explain how the procedure has been modified to enrich for the number of plaques with the mutant gene.
- PLEASE ANSWER BOTH PARTS OF THE QUESTION a.) The Question on the image b.) From this experiment, in how many genes have we identified mutations?Describe the mutation that created the HBs allele: type of mutation, location of mutation on HbA sequence (#of bases from beginning of sequence), nucleotide change (from which base to which base?) Also mention the reputable published source to support this informationBriefly explain why RNA-seq gives more information about the transcriptome than does microarray analysis. Read Tools of Biochemistry 24A before answering this question.