The completely synthetic yeast chromosome Syn IIIcontains a loxP site in the 3′ UTR of every gene thatis potentially nonessential to yeast survival. As youwill recall from Chapter 6, loxP sites are targets ofsite-specific recombination. The researchers who constructed Syn III included these loxP sites as a way to“scramble” the chromosome, meaning that parts ofthe chromosome could easily be deleted or rearranged.The goal of these investigations is to drive the evolution of Syn III so as to define a minimal genome thatcan support the life of this organism. Outline the experiment the researchers would do to scramble Syn IIIin order to define a minimal genome.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
The completely synthetic yeast chromosome Syn III
contains a loxP site in the 3′ UTR of every gene that
is potentially nonessential to yeast survival. As you
will recall from Chapter 6, loxP sites are targets of
site-specific recombination. The researchers who constructed Syn III included these loxP sites as a way to
“scramble” the chromosome, meaning that parts of
the chromosome could easily be deleted or rearranged.
The goal of these investigations is to drive the evolution of Syn III so as to define a minimal genome that
can support the life of this organism. Outline the experiment the researchers would do to scramble Syn III
in order to define a minimal genome.
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