The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis of short tandem repeats, STRs, in the human genome. This approach has many distinct advantages. Please list and explain three of those advantages.
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The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis of
short tandem repeats, STRs, in the human genome. This approach has many distinct advantages.
Please list and explain three of those advantages.
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- DNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of these methods and the materials required to perform each of these molecular techniquesMicroarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein technologies. Describe in detail, the components of a PCR reaction and why they are required
- In a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y and Brother X, there is a sample of DNA from a crime scene that is likely to belong to the guilty party. Based on the gel photo below, which shows the results of an electrophoresis gel following PCR amplification at one locus of 5 DNA samples - one crime scene sample and 4 suspects - which suspects can be excluded from this investigation? [Keep in mind that it is not possible for a heterozygous person to leave only one allele at a crime scene. If any one allele does not match, then that suspect is eliminated.] choose all that applyWhat are STRs, and why are they useful for DNA profiling?As a technique for detecting genetic variations, RFLP has substantial drawbacks. Name one such drawback, explain why it is unique to RFLP analysis with specific reference to the technique, and discuss why DNA sequencing overcomes this drawback. Please leave the link for any sources used. Thanks!
- What is the useful of the Development of highly sensitive and low-cost DNA agarose gel electrophoresis detection systems, and evaluation of non-mutagenic and loading dye-type DNA-staining reagents in modern medicine today?Why do you think the DNA is stored cold with the InstaGene matrix after boiling the samples and during subsequent steps in DNA Abstraction of PCR Bioinformatics?what are the advantages and disadvantages of Gel electrophoresis in DNA analysis
- During PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)The exponential nature of PCR allows spectacular increases in the abundanceof a DNA sequence being amplified. Consider a 10-kbp DNA sequence in agenome of 1010 base pairs. What fraction of the genome does this sequence represent? That is, what is the fractional abundance of this sequence in this genome?Calculate the fractional abundance of this target sequence after 10, 15, and 20 cycles of PCR, starting with DNA representing the whole genome and assuming that no other sequences in the genome undergo amplification in the process.In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in buffer?