The key to biopolymer self-replication is:
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- In the isolation of DNA, which of the following is the function of chilled ethanol? a. chelate the Mg2+ ions that are essential for the overall integrity of the cell membrane b. break down cell membrane thus releasing intracellular structure c. precipitate the nucleoproteins through salting-out d. retard the nuclease activity then precipitate the DNAWe want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNADNA gyrasea. promotes negative supercoiling.b. relaxes positive supercoils.c. cuts DNA strands as part of its function.d. does all of the above
- The Nucleosome Is the Fundamental Unitof _____________PackagingA bacterial chromosome typically containsa. a few thousand genes.b. one origin of replication.c. some repetitive sequences.d. all of the above.Gel electrophoresis separates the DNA fragments according to ____. a. their type b. their stage in replication c. the orientation of the nucleotides in the fragment d. their size e. their ability to bind to the gel
- Synthesis of new molecules of deoxyribonucleic acid is the process of A. replication B. translation C. transcription D. ossification E. cytokinesis1. Biolistics: plant tissues; heat shock treatment: _______ a. mammalian cells b. viruses c.bacteria d.animal cells 2. This genetic engineering process involves the incubation of the purified DNA with the selected RE, at optimal conditions for that specific enzyme. a. Ligation b. Cutting c. Transformation d. IsolationTransfer of F factor via bacterial conjugation uses a molecular mechanism related to: a. Transcription b. Telomere replication c. Theta replication d. Rolling circle replication e. None of these is correc
- DNA replication requires ________ . a. DNA polymerase c. primers b. nucleotides d. all are requiredShow the structure of a DNA where the lead strand is ATCG. Show H-, glycosidic, phosphoester bondings.4. Differentiate replication, transcription and translation by describing the changes which occur in each process.A protein is a glycoprotein and it is active when it is glycosylated. The gene of this protein was cloned in a bacteria and the recombinant protein was produced but it was inactive the reason is a.bacteria do not have mitochondria b.bacteria does not have an ER c.bacteria does not have a nucleus
