The following experimental values were obtained for a 1 x 10 M p-nitrophenylphosphate (S) solution, TRIS buffer, and 0.2 ml of enzyme. DETERMINE the Michaelis-Menten constant. ml de S 4 ml TRIS 1 2 Ro (abs/min) 0.0313 0.05 0.0375 Abs a too 1.37 1.05 0.79

The following experimental values were obtained for a 1 x 10 M p-nitrophenylphosphate (S) solution, TRIS buffer, and 0.2 ml of enzyme. DETERMINE the Michaelis-Menten constant. ml de S 4 ml TRIS 1 2 Ro (abs/min) 0.0313 0.05 0.0375 Abs a too 1.37 1.05 0.79

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter9: Aqueous Solutions And Chemical Equilibria

Section: Chapter Questions

Problem 9.8QAP

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Question

The following experimental values were obtained for a 1 x 10 M p-nitrophenylphosphate (S)
solution, TRIS buffer, and 0.2 ml of enzyme. DETERMINE the Michaelis-Menten constant.
ml de S
4
3
ml TRIS
2
Ro (abs/min)
0.0313
0.05
0.0375
Abs a too
1.37
1.05
0.79

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