The obligate anaerobic bacterium Clostridium perfringens dies when exposed to air. What would predict as the result if you performed a catalase test on this bacterial species? Explain why.
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1. . The obligate anaerobic bacterium Clostridium perfringens dies when exposed to air. What would predict as the result if you performed a catalase test on this bacterial species? Explain why.
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- 1. An organism that has peroxidase and superoxide dismutase but lacks catalase is most likely an2. A mannitol-salt agar plate was inoculated with these bacteria and is shown below. A. What type of organisms grow on this medium? B. Based on the reaction below, what can you say about the organism derived from the patient's abscess?1. What results are expected if you drop H2O2 into a cut on your arm? Explain why those results are expected. 2. To do this test, why do you have to grow the organism in medium containing tryptophan? 3. Why is a red color after the addition of zinc a negative result for nitrate reductase?
- Catalase: 1. Explain the incubation conditions 2. Explain the reagents being added 3. Explain the observations in Catalase 4. Explain the interpretations in Catalase1. Escherichia coli but not Pyrolobus fumarii will grow at 40°C, while P. fumarii but not E. coli will grow at 110°C. What is happening (or not happening) to prevent growth of each organism at the nonpermissive temperature?1. How can zinc dust differentiate nitrate reductase positive- and nitrate reductase negative organisms? Answer this comprehensively.
- 1. Clostridium butyricum does not appear to have superoxide dismutase (SOD) and catalase enzymes. How would the growth of C. butyricum in oxic conditions compare to its growth in anoxic conditions? On this basis, what is the aerotolerance category of C. butyricum? 2. Citrobacter freundii is a facultatively anaerobic bacterium. Why would you expect this bacterium to grow better (i.e. form larger colonies) in an oxic environment than in an anoxic one? (as a hint, do some research on the “Pasteur effect”)The results of Figure 2 highlights the antibacterial activity of cacay oil. Since the method involves adding cacay oil to a "broth" medium, it can be inferred that cacay oil is hydrophilic. Agree or Not?Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours. Which of the following can be interpreted from this result? A. The organism did not grow so lysine decarboxylase activity could not be determined. B. The organism is negative for lysine decarboxylase. C. The media is too acidic to determine lysine decarboxylase activity. D. The organism is positive for lysine decarboxylase
- Clostridium tetani is a common soil bacterium and causes tetanus. Would you expect tetani to possess the enzyme catalase? Explain. Research on the growth requirements of this bacterium is required.1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.1. Define the following terms: a. Chermolithoheterotroph b. Microaerophile c. Chermoorganoheterotroph d. Obligate anaerobe e. Facultative anaerobic f. Coliform