The pH values of the different compartments are shown below: Matrix Intermembrane space Cytosol pH 7.8 – 8.0 pH ~ 7.0 pH 7.0 – 7.4 How can you explain these values in terms of what is known about mitochondrial function?
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c) The pH values of the different compartments are shown below:
Matrix Intermembrane space Cytosol
pH 7.8 – 8.0 pH ~ 7.0
pH 7.0 – 7.4
How can you explain these values in terms of what is known about mitochondrial function?
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- Which of the following statements is a lie? Select one: a.The formation of water aids in establishing the ion gradient between the inner matrix and intermembrane space. b.Every protein complex in the electron transport chain is involved in the direct pumping of H+ in the intermembrane space. c.There is an existing electrochemical gradient between the intermembrane space and the inner mitochondrial matrix.It has been estimated that mitochondria occupy 20% of the volume in the human body. For a 70- kg adult, the average number of mitochondria has been estimated to be 1 × 10 (10,000 trillion). Using this information, provide a rough estimate of the average mass of a mitochondrion.A principle of biology is that structure determines function.Explain how the invaginations of the inner mitochondrialmembrane are related to mitochondrial function.
- Energy-consuming active transport is used to move protons in all of the following directions except: A. from the mitochondrial matrix, across the mitochondrial cristae, into the intermembrane space B. from the extracellular fluid, across the plasma membrane, into the Halobacterium halobium cytosol C. from the cytosol of plant cells, across the vacuolar membrane, into the central vacuole D. from the cytosol of skeletal muscle cells, across the plasma membrane, into the extracellular fluid E. from the chloroplast stroma, across the thylakoid membrane, into the thylakoid spaceThe following are true of the mitochondrial structure I. The inner mitochondrial membrane is convoluted to give more space for oxidative phosphorylation and ATP synthesis. II. The porins in the outer membrane makes the outer membrane less permeable than the inner membrane. III. The matrix contains the machinery for gene expression. IV. The intermembrane space is between the outer and inner membrane of the mitochondrion. I, II, III, IV I, II, III only I, III, IV only I, II, IV onlyThe difference in pH between the internal and external surfaces of the inner mitochondrial membrane is 1.4 pH units (external side acidic). If the membrane potential is 0.06 V (inside negative), what is the free energy change on transporting 1 mol of protons across the membrane from outside to inside at 25°C?
- Fill in the bold using the table. Because the inner mitochondrial membrane is impermeable to L, when there is an excess of mitochondrial L, it converts to M using a TCA condensation reaction, M crosses to the cytosol using a designated transport system. In the cytosol, M reconverts to L and oxaloacetate. Oxaloacetate is subsequently converted to malate with simultaneous production of N, a prerequisite cofactor for the glycolysis pathway. This reaction is then followed by conversion of N to pyruvate which produces O, a prerequisite cofactor for the fatty acid synthesis pathway. L M N O A. ATP pyruvate malate NADPH B. ATP citrate ATP NAD+ C. acetyl CoA pyruvate NAD+ NADH D. acetyl CoA citrate NAD+ NADPH E. glucose pyruvate malate NADP+Assuming that the pH of the IMS is 1.4 units lower than the pH of the matrix, the free energy yield associated with the transport of a proton from the mitochondrial intermembrane space (IMS) to the mitochondrial matrix is △ G of the reaction is 8.310 KJmol-1 What is the minimum number of protons that must enter the mitochondrial membrane from the IMS in order to synthesize one molecule of ATP?In human cells, where does fatty acid elongation (that is, beyond the C16:0 normally produced by fatty acid synthase) occur? a)On the outer (cytosolic) face of the outer mitochondrial membrane b)On the outer (cytosolic) face of the endoplasmic reticulum membrane c)Free in the cytosol d)In the mitochondrial matrix
- Although the outer mitochondrial membrane is permeable to all small molecules, the inner mitochondrial membrane is essentially impermeable in the absence of specific transport proteins. Consider this information answer: The ATP generated by oxidative respiration is used throughout the cell. The majority of ATP production occurs in the mitochondrial matrix. How do you think ATP is made accessible to enzymes in the cytosol and other organelles?The fatty acid side chains of the phospholipids in the inner mitochondrial membrane exhibit a large proportion of multiple unsaturations. As a result, will the membrane be hydrophobic or hydrophilic? Also will it be more fluid or more rigid? How do these characteristics help explain the observation that the inner membrane is impermeable to ions such as Mg2+ and Cl–? Hint: what moves these ions through the membrane?What would be the effect on ATP production of decreasing the pH inside the mitochondrial matrix? Explain