The pKa of HEPES is 7.55 at 20 degrees Celsius, and its molecular weight is 238.1. Calculate the mass of HEPES and the volume of 1.0 M NaOH that would be needed to make 300mL of 0.2 HEPES buffer at pH 7.2

Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter16: Reactions Between Acids And Bases
Section: Chapter Questions
Problem 16.54QE
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The pKa of HEPES is 7.55 at 20 degrees Celsius, and its molecular weight is 238.1. Calculate the mass of HEPES and the volume of 1.0 M NaOH that would be needed to make 300mL of 0.2 HEPES buffer at pH 7.2

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