The rate of reaction can be determined by measuring the change in optical rotation of the sample as a function of time if a reactant or product is chiral. This technique is especially useful for kinetic studies of enzyme catalysis involving sugars. For example, the enzyme invertase catalyzes the hydrolysis of sucrose, an optically active sugar. The initial reaction rates as a function of sucrose concentration are as follows: a. Construct a Lineweaver-Burk plot for these data. [sucrose]0 (M) 0.029 0.059 0.088 0.117 0.175 0.234 Rate (M s−1) 0.182 0.266 0.310 0.330 0.362 0.361 b. Determine the Michaelis constant for invertase.
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
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a. Construct a Lineweaver-Burk plot for these data.
|
[sucrose]0 (M) |
0.029 |
0.059 |
0.088 |
0.117 |
0.175 |
0.234 |
|
Rate (M s−1) |
0.182 |
0.266 |
0.310 |
0.330 |
0.362 |
0.361 |
b. Determine the Michaelis constant for invertase.
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