The secondary antibody used in the laboratory for western blotting is conjugated to which enzyme?   alkaline phosphate    horseradish peroxide    GAPDH   alkaline decarboxylase

Q: To probe for presence tubulin in protein samples by western blot you use a anti tubulin monoclonal…

A: A defense system in the body of an individual which constitutes a complex network of cells and…

Q: If you gain immunity to an antigen by receiving a vaccine this is:     Natural passive…

A: Immune system is system which helps our body to fight against the foreign substances which will…

Q: With regard to the experiment described in Figure, The DNA extract was treated with DNase, RNase, or…

A: Mutation is defined as the sudden inheritable change that occurs in the DNA sequence.  It is caused…

Q: Which of the following antigen-antibody reaction is used in blood ?grouping Precipitation O…

A: Blood is thick fluid that circulates through out the body in order to supply oxygen and nutrients to…

Q: Antigen Antibody Enzyme-labelled antibody Substrate This image represents a/an. direc ELISA sandwich…

A: Enzyme-linked immunosorbent assay (ELISA) is an immunological assay that is used in laboratories to…

Q: Ascorbic acid prevents: Select one: Scurvy Rickets Pellagra Antibody synthesis

A: A vitamin is an organic molecule that is an essential micronutrient which an organism needs in small…

Q: An antibody consists of four protein chains, two______chains and two_______ chains. Each is composed…

A: The molecules bind to one of the two types of protein receptors of the immune system. One of the…

Q: Would it be worse for the function of the antibody for it to lose the Fc region or a Fab region?…

A: The  N-terminal fragment square degree is called the beautiful space, and also the C-terminal…

Q: w..*** Antibodies Select one: a. are shaped like the letter B b. assist in destroying particular…

A: An antibody (Ab), otherwise called an immunoglobulin (Ig), is a huge, Y-molded protein utilized by…

Q: A pathogenic bacteria has entered the body via a wound: A few hours later, antigen presenting cells…

A: Endogenous antigens are presented by class I MHC while exogenous are presented by class II MHC. Let…

Q: I| HO CH,OH CH;ČNH Protein в A II CH2OH HO ČH4OH OH IV Is the antigenic determinant reducing or…

A: Glycans:  These are chain-like structures made up of monosaccharides (single sugar molecules)…

Q: To detect a toxin in food, the contaminated food is normally injected into at least two guinea pigs.…

A: The Humoral immune response in the body is regulated by the B cells which produce antibodies against…

Q: The conjugated -antibody can be either primary antibody or the secondary antibody True False

A: Primary antibody binds to antigen while secondary antibodies bind to primary antibody Fc region. Fc…

Q: When a patient goes from testing negative for a specific antibody to testing positive a month later…

A: An antibody or serology test is a blood test that looks for signs of a previous infection. It…

Q: A monomer of an immunoglobulin molecule has all the following except: O 1) One Fab fragment 2)…

A: A monomer immunoglobulin has more than one antigen-binding site and hence the correct option is (3)

Q: Which

A: Introduction :- The sandwich enzyme linked immunosorbent assay(ELISA) is a Sensitive and robust…

Q: Which of the following is NOT true about the production of monoclonal antibodies? what option below…

A: Antibody : An antibody is a protein that sticks to a specific protein called an antigen.  Antibodies…

Q: Which of the following are elements of the structure of antibodies? Select all that apply. The…

A: It is a large Y shaped protein used by the immune system to identify and neutralize foreign objects.…

Q: In an immunoblot the primary antibody recognizes:   the secondary antibody    nitrocellulose…

A: Ans. Immunoblot- It is also known as the western blotting technique, a highly sensitive method that…

Q: The first antibody used in an immunoassay to detect the foreign particle     antigen     enzyme…

A: Detection of a specific protein can be done by immunoassay techniques. The specific proteins that…

Q: A monoclonal antibody molecule has only one Fab domain, while a polyclonal antibody has two Fab…

A: An Antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly…

Q: Write role of SDS-PAGE in monoclonal antibodies production

A: SDS-PAGE is a gel electrophoresis technique that separates the proteins based on the mass of the…

Q: Anti-A antibody will react with cells with: a. A-Antigen O b.B-antigen OC. C-antigen d. D-antigen

A: Blood is a liquid substance which flows in the blood vessels and carry nutrients and oxygen to the…

Q: Differentiate between the serological tests used to identify isolatedcultures of pathogens and those…

A: Serologic tests are blood tests done for antibodies in the blood. They can involve a number of…

Q: that can chemically antagonize the toxic agent. Which of the following drugs is most likely being…

A: Cardiac glycosides are a class of drugs that is used to treat heart failure in patients. Cardiac…

Q: The concentration (or titer) of antibody to a specific antigen can be determined using a(n) test.…

A: Introduction :- Antigen (Ag) is a molecule or molecular structure, as well as any foreign…

Q: antibody surface involved in antigen binding varies depending on the size and nature of the antigen.…

A: Antibody: These are the Y shaped protein molecule which has capacity to bind or react with the…

Q: You are seeing a 4-year old girl who has a 6-week history of recurrent left knee pain and a limp. On…

A: Positive result of a test shows the presence of the disease while negative test confirms the absence…

Q: Which class of antibodies functions as a dimer?   IgE IgG IgM Secretory IgA

A: Immunoglobulins or Ig molecules are synthesized and released by the B-lymphocytes or B-cells. It is…

Q: Choose the correct match. Blood group A - Antigen A, Antibody B Blood group A- Antibody A, Antigen B…

A: ABO blood group is the most used blood group system for the classification of blood groups. It is…

Q: hich portion of an antibody provides antigen binding sites? A.Heavy chain B.Constant portion…

A: The very first antibody was discovered in the year 1890 by  Emil von Behring.

Q: O Antibody interference occurs when (multiple possible answers) Choose all that apply. O free…

A: In immunoassays, the interaction between the contents in the sample with one or more antibodies is…

Q: hoose all that are correct about indirect ELISA. A) An antigen can bind the substrate B) A…

A: ELISA is also called as the enzyme linked immunosorbent assay. It is an assay technique. This…

Q: labeled secondary

A: Antibodies are the proteins (immunoglobins) produced by B-Lymphocytes. There are two groups of…

Q: hinge region Fc Fab kappa alpha chain Drag answer here Drag answer here. Drag answer here Drag…

A: The structure of the antibody is Y shaped with four polypeptide chains with two heavy and two light…

Q: You want to detect troponin in an immunoblot. Give an example of a potential primary rabbit antibody…

A: Immunoblotting is a sensitive and rapid assay to detect and characterize proteins. It works by…

Q: Click on the region of the antibody responsible for binding to antigens.

A: In this question, we have to show the site for antigen binding in antibody.

Q: n immunoassay what is a secondary antibody? Antibodies used to recognize antigens. They confer…

A: .Antibodies are specific to the antigen. in an immunoassay specific antibodies are created which…

Q: Which one is true and which one is false?  SDS PAGE gel can be prebed with NBT/BCIP to perform an…

A: Polyacrylamide gel is the crosslinked polymer of bis-acrylamide and acrylamide polymerized by…

Q: Which of the following is the most antigenic? O Carbohydrates O Nucleic acids O Proteins O Lipids

A: Antigen It is defined as a molecule which is involved in initiating the production of an antibody.…

Q: Write down the steps followed in monoclonal antibodies production.

A: Antibodies --( Immunoglobulin)-- Antibodies , a specialized immune protein develop inside the body…

Q: Which type of hypersensitivity involves solubleantigen-antibody complexes?a. type Ib. type IIc. type…

A: The undesirable reactions that are produced by the immune system and include autoimmunity and…

Q: The valence of an antibody that is a pentamer is: 1) 2 O 2) 4 3) 3 4) 10

A: The higher an antibody valency which is the number of antigen binding sites, the higher the amount…

Immunology

Immunology is the division of science involved with the immune reactions occurring in living organisms. The organisms are accustomed to evolving internal defense mechanisms to evade the invasion of microbial pathogens. Diverse cells are connected with stimulating the immune responses, which produce inhospitable conditions to the microbes prompting their lysis and thus inhibits the multiplication of the pathogens. The immune system can distinguish the pathogens through the aid of cellular intercommunication and launch responses to destroy them.