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- In agglutination reactions, the antigen is a……… in precipitation reactions, the antigen is a……………The sandwich type of ELISA detects patient antibodies to an antigen.A. FalseB. TrueThe direct ELISA test requires only one antibody only one antigen both primary and secondary antibody only secondary antibody conjugated with enzyme system
- All of the following are used in a direct ELISA, except: A) Primary enzyme linked antibody B) Substrate C) Secondary enzyme linked antibody D) Primary antibodyThe substrate For the secondary antibody used in the lab for western blothing is : TMB TritonX-100 tween BCIP/NBTIn a direct ELISA, which of the following does the patient provide? A.) Substrate B.) Enzymes C.) Antibodies D.) Antigens
- An ELISA test is useful for detecting the presence of antigens but not antibodies in a sample. true or falseThe secondary antibody used in the laboratory for western blotting is conjugated to which enzyme? alkaline phosphate horseradish peroxide GAPDH alkaline decarboxylaseDisrobe laboratory method Western Blot used to evaluate the immune response, describe how it works in detail, Also, please provide at least one pathogen your method of analysis is used to detect.
- A positive ELISA assay usually results in __________. decreased motility fluorescence agglutination a color change visible bubblingCounterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingIn general, protein detection methods are based on: a. fluorescence b. column affinity c. enzyme-substrate reaction d. antigen-antibody reaction