The student used an eyepiece graticule to calculate the size of some of the onion cells. Figure I shows the student's eyepiece graticule and stage micrometer. The stage micrometer measures in millimetres. 0 mm 0 Figure 1 10 20 30 the slide. 40 Eyepiece graticule 2 Stage micrometer Use Figure 1 to calculate the size of one division on the student's eyepiece graticule, in micrometres. Show your working.
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- A student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometersFor what purpose would you adjust each of the following microscopecomponents during a microscopy exercise?a. Iris diaphragm:b. Coarse-adjustment knob:c. Fine-adjustment knob:d. Condenser:e. Mechanical stage control:Contrast how the views differ under high power (40x) and oil immerson (100x) objective lenses in terms of a) magnification b) detail of the cell, c) size of the area being viewed and d) resolution.
- Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer choices camera detector monochromator light sourcea. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?a. Why must you slowly turn the fine adjustment knob? b. Is natural light or is artificial light used when using the microscope?
- which objective lens is in place if the object you are viewing is magnified 1000x? (assume ocular lens magnification = 10x)What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.You can see your cells using the scanning and the 10x objective lenses. You cannot focus on anything when you add oil to your slide and move to the oil immersion objective. Your instructor comes over and wipes off your oil, turns over the slide, adds new oil, and moves the oil immersion objective into place. You can now focus on your cells. Knowing how immersion works, why couldn't you see the cells when the slide was upside down?
- The girl is focusing a slide and she is turning the coarse adjustment knob up toward the slide. A. What has happened to the slide? B. Is this the proper way to focus? C. Should you focus by turning the coarse adjustment up or down? D. What happens to a microscope if place at the edge? E. What should you do to present this from happening?If we used the 365nm setting on the UV light box would this have caused more or less UV light damage, explain why?How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity