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There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure out which baby goes with each set of parents. You started to look at the repeat sequence Q2. of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can have different numbers of repeats (i.e. “n" shown below can vary). 5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3' 3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5' a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of each primer's sequence) which you would use for PCR reaction to amplify the repeat sequence. Make sure to label the ends (5' and 3' of each primer). You obtain the following results. markers dad#1 mom#1 dad#2 mom#2 dad#3 mom#3 BabyA BabyB BabyC n = 50 n = 45 n = 40 n = 35 n = 30 n = 25 n = 20 | | b) Why is it that having more repeats leads to a band that is higher in the gel? c) Why is it that some people show only one band? d) Why is it that some people show two bands? e) Given the data so far, for which of the three babies (only Baby A, only Baby B, or only Baby C, all of them, or none of them) can you already conclusively tell who the parents are? ) If you amplify a DNA sequence through PCR, what are the 4 reaction components that you would absolutely need? Provide names only.

There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure out which baby goes with each set of parents. You started to look at the repeat sequence Q2. of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can have different numbers of repeats (i.e. “n" shown below can vary). 5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3' 3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5' a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of each primer's sequence) which you would use for PCR reaction to amplify the repeat sequence. Make sure to label the ends (5' and 3' of each primer). You obtain the following results. markers dad#1 mom#1 dad#2 mom#2 dad#3 mom#3 BabyA BabyB BabyC n = 50 n = 45 n = 40 n = 35 n = 30 n = 25 n = 20 | | b) Why is it that having more repeats leads to a band that is higher in the gel? c) Why is it that some people show only one band? d) Why is it that some people show two bands? e) Given the data so far, for which of the three babies (only Baby A, only Baby B, or only Baby C, all of them, or none of them) can you already conclusively tell who the parents are? ) If you amplify a DNA sequence through PCR, what are the 4 reaction components that you would absolutely need? Provide names only.

Biology Today and Tomorrow without Physiology (MindTap Course List)
Biology Today and Tomorrow without Physiology (MindTap Course List)
5th Edition
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Christine Evers, Lisa Starr
Chapter7: Gene Expression And Control
Section: Chapter Questions
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please only answer parts d and e and f

There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure out which baby goes with each set of parents. You started to look at the repeat sequence Q2. of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can have different numbers of repeats (i.e. “n" shown below can vary). 5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3' 3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5' a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of each primer's sequence) which you would use for PCR reaction to amplify the repeat sequence. Make sure to label the ends (5' and 3' of each primer). You obtain the following results. markers dad#1 mom#1 dad#2 mom#2 dad#3 mom#3 BabyA BabyB BabyC n = 50 n = 45 n = 40 n = 35 n = 30 n = 25 n = 20 | |
Transcribed Image Text:There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) You decided to perform PCR to figure out which baby goes with each set of parents. You started to look at the repeat sequence Q2. of 5'TTAGGAT3' that is located on chromosome #15. Note that different humans can have different numbers of repeats (i.e. “n" shown below can vary). 5'.GCTAAGTATTGCTCAAGA(TTAGGAT)n...GATAAATAACTGGCTAGTA...3' 3'..CGATTCATAACGAGTTCT(AATCCTA)n...CTATTTATTGACCGATCAT...5' a) Write the sequence of Primer 1 and Primer 2 (only the first 4 nucleotides of each primer's sequence) which you would use for PCR reaction to amplify the repeat sequence. Make sure to label the ends (5' and 3' of each primer). You obtain the following results. markers dad#1 mom#1 dad#2 mom#2 dad#3 mom#3 BabyA BabyB BabyC n = 50 n = 45 n = 40 n = 35 n = 30 n = 25 n = 20 | |
b) Why is it that having more repeats leads to a band that is higher in the gel? c) Why is it that some people show only one band? d) Why is it that some people show two bands? e) Given the data so far, for which of the three babies (only Baby A, only Baby B, or only Baby C, all of them, or none of them) can you already conclusively tell who the parents are? ) If you amplify a DNA sequence through PCR, what are the 4 reaction components that you would absolutely need? Provide names only.
Transcribed Image Text:b) Why is it that having more repeats leads to a band that is higher in the gel? c) Why is it that some people show only one band? d) Why is it that some people show two bands? e) Given the data so far, for which of the three babies (only Baby A, only Baby B, or only Baby C, all of them, or none of them) can you already conclusively tell who the parents are? ) If you amplify a DNA sequence through PCR, what are the 4 reaction components that you would absolutely need? Provide names only.
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