You have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are 500bp and 1000bp in length. Tube #2 has fragments that are 7500bp and 8000bp in size. If you were to perform agarose gelelectrophoresis and run the contents of each tube in two separate lanes on the same gel, what would you expect tosee?O That the difference between the distances migrated by the two fragments in Tube #1 was much greater than the differencebetween the distances migrated by the two fragments in Tube #2.O That the difference between the distances migrated by the two fragments in Tube #1 was the same as difference between thedistances migrated by the two fragments in Tube #2.O That the difference between the distances migrated by the two fragments in Tube #1 was much less than the difference betweenthe distances migrated by the two fragments in Tube #2.O It is not possible to estimate what we would expect to see.

Electrophoresis Electrophoresis is used in biological laboratory to separate different size of DNA,…

You have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are 500bp and 1000 bp in length. Tube #2 has fragments that are 7500bp and 8000bp in size. If you were to perform agarose gel electrophoresis and run the contents of each tube in two separate lanes on the same gel, what would you expect to see?

You have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are 500bp and 1000 bp in length. Tube #2 has fragments that are 7500bp and 8000bp in size. If you were to perform agarose gel electrophoresis and run the contents of each tube in two separate lanes on the same gel, what would you expect to see?

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1CT

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Similar questions

Which of the DNAs shown in Figure would move fastest during agarose gel electrophoresis?
If a 1000 bp of DNA were inserted between the two restriction sites, how would the banding pattern on the gel differ from the one you drew in part a? (PART A WITH THE FIRST PART OF THE QUESTION IS ATTACHED)
During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricity
If a 500 bp of DNA between the two restriction sites were deleted, how would the banding pattern on the gel differ from the one you drew in part a? (PART A WITH THE FIRST PART OF THE QUESTION IS ATTACHED)
A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2% agarose gel instead, what would you expect would happen to the migration rate of DNA and why?
When DNA replication was investigated by using heavy, N15 DNA to mark the original molecules, and light, N14 DNA to mark the newly synthesized molecules, one band was found in the middle of the centrifuge column after one round of replication, and two bands were found (middle and top of column) after 2 rounds of replication. Imagine that after 1 round of replication 2 bands were found, one at the bottom and one at the top of the centrifuge column. In that case, what model of DNA replication would have been supported? The dispersive model The conservative model The Franklin model The semi-conservative model
Referring to Figure 7-20, answer the following questions:a. What is the DNA polymerase I enzyme doing?b. What other proteins are required for the DNApolymerase III on the left to continue synthesizingDNA?c. What other proteins are required for the DNApolymerase III on the right to continue synthesizingDNA?
A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than one with 2,500 bp.True or false?
Based on your gel, how big are the DNA fragments in each lane? Enter your results in Table 2.
How would you expect your results to change if the enzyme did not have time to cut all of the sites? (Hint, remember that there are many pieces of the lambda DNA in the tube – draw yourself a simplified model where there are four restriction sites, and imagine what would happen if the enzyme only cut at one, two, three or all four, randomly, each time). How would you expect your results to change if you treated them with both EcoRI and HindIII in the same tube? Why might it be safer for the virus to have its DNA become circular when it enters the host, rather than remain linear?
How are DNA molecules visualized in a gel after electrophoresis? Why do DNA molecules migrate toward the + electrode? What determines the rate of their migration? What is the effect of PEG on DNA fragments of different sizes? How is this influenced by the concentration of PEG?
When proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?