There may be more than one correct answer, select all that apply. To clone genes, a plasmid vector should contain a(n) a.) telomeres b.) orgin of replication c.) antibiotic resistance gene d.) restriction endonuclease cut site The CRISPR/Cas system has revolutionized the efficiency at which scientists can experimentally induce a mutation in a gene to investigate the functional role of the protein it encodes. Which of the following are required in the CRISPR/Cas system? Cas9 nuclease Bacterial genome Access to target gene Guide RNA complementary to gene of interest
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
There may be more than one correct answer, select all that apply.
To clone genes, a plasmid vector should contain a(n)
a.) telomeres
b.) orgin of replication
c.) antibiotic resistance gene
d.) restriction endonuclease cut site
The CRISPR/Cas system has revolutionized the efficiency at which scientists can experimentally induce a mutation in a gene to investigate the functional role of the protein it encodes. Which of the following are required in the CRISPR/Cas system?
- Cas9 nuclease
- Bacterial genome
- Access to target gene
- Guide RNA complementary to gene of interest
Introduction
A plasmid vector is a small, circular piece of DNA that is used as a tool for transferring genes from one organism to another. It is commonly used in genetic engineering and biotechnology to introduce a specific gene into a host organism, such as bacteria, yeast, or animal cells. The plasmid vector contains the gene of interest, as well as other components that allow for the gene to be replicated and maintained within the host.
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