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Think about the structure and transmission of F factors and discuss
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- . Figure 1-7 shows a simplified pathway for argininesynthesis in Neurospora. Suppose you have a specialstrain of Neurospora that makes citrulline but not arginine. Which gene(s) are likely mutant or missing inyour special strain? You have a second strain ofNeurospora that makes neither citrulline nor argininebut does make ornithine. Which gene(s) are mutant ormissing in this strain?Bt eggplant is a genetically-modified eggplant developed by Filipino scientists. It was developed to become pest-resistance containing the gene of the bacterium Bacillus thuringiensis which contains toxins that are lethal to some insects including the pest bollworm. Currently, it is under controversy because of a legal battle between its supporters and those who are against it. Should the government ban the production, commercialization and consumption of the said product?Looking at the sequence of gene X, you hypothesize that there is an intrinsic terminator in the 3' end of gene X. To test your hypothesis, you delete the putative intrinsic terminator (AIT). Which of the following western blots for gene X would you expect if your hypothesis is correct (that the IT sequence is an intrinsic terminator). O O wild-type ΔΙΤ O Blot A O O A Blot C Blot E Blot B O Blot D wild-type B wild-type AIT C AIT wild-type D ΔΙΤ wild-type E
- Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways, pancreas, and sweat glands. Mutations in both copies of the CFTR gene causes cystic fibrosis. Imagine that you have sweat gland samples from several Cystic Fibrosis patients (A-C) with unknown mutations in CFTR. You also have normal (+) sweat gland sample to use as a positive control. А В С А В С Choose which mutation would explain the RNA and protein results in A, B, & C: 1. Promoter/Regulatory mutation 2. Silent mutation 3. Missense mutation 4. Deletion mutation 5. Splice site mutation 6. Nonsense mutation RNA gel Protein gelThe following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dg+ = 10% ad g+= less than 1% What is the map distance between the a and d genes? 6 map units less than 1 map unit 10 map units 84 map units 74 map unitsThe following recombinants are recovered when conjugation occurs between an a*d*g+ donor and an adg recipient. at d+ g+ = 84% a d g+ = 6% at d g+ = 10% a dt g+ = less than 1% What is the map distance between the a and d genes? 10 map units 74 map units less than 1 map unit 84 map units 6 map units
- Based on the NCBI Blastp and reciprocal analysis, are there any mutations to suggest that mus musculus is a good model to test the human FGFR3 gene. This is the only model we have to go off of to predict whether a mouse is suitable. I can’t seem to grasp what exactly I’m suppose to look for in these two photos.To produce transgenic plants, plant tissue is exposed to Agrobacteriumtumefaciens and then grown in media containing kanamycin, carbenicillin, and plant growth hormones. Explain the purpose behind each of these three agents. What would happen if you left out the kanamycin?Of all the genes in the human genome, the ones withthe most characterized Alu insertions are those thatcause hemophilia, including several insertions in thefactor VIII and factor IX genes. Based on this fact, yourcolleague hypothesizes that the Alu element prefers toinsert into these genes. Do you agree? What other reason can you provide that also explains these data?
- By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the table:Draw a map that shows the order of genes and the locations ofthe origins of transfer among these different Hfr strains?Describe a series of experiments to SELECT and/or SCREEN for strains containing mutations that specifically disrupt the function of StyRS/StyAB and not StyC/StyD in Pseudomonas strain Y2 .In the experiment of Figure shown, Lederberg and Tatum could notdiscern whether met+ bio+ genetic material was transferred to themet− bio− thr+ leu+ thi+ strain or if thr+ leu+ thi+ genetic materialwas transferred to the met+ bio+ thr− leu− thi− strain. Let’s supposethat one strain is streptomycin-resistant (say, met+ bio+ thr− leu−thi−) and the other strain is sensitive to streptomycin. Describe anexperiment that could determine whether the met+ bio+ geneticmaterial was transferred to the met − bio− thr+ leu+ thi+ strain orthe thr+ leu+ thi+ genetic material was transferred to the met+ bio+thr− leu− thi− strain. bio+ thr− leu− thi− strain.