If an E. coli auxotroph X could grow only on a medium containing leucine, and an auxotroph Y could grow only on a medium containing threonine. (i) Write the genotype of both E. coli strains. (ii) How would you test whether DNA from X could transform Y?

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Q: bacteria

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Q: From the data, what can you conlucde about the effect of this mutant?

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Q: Briefly explain in situ hybridization, giving some applications of this technique.

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Q: Why is replica plating used to isolate an auxotrophic mutant from a prototrophic parent?

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Q: How does an auxotroph differ from a prototroph in bacteria?

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Q: How Hfr strains allow genetic mapping of the E. coli chromosome ?

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Q: How is an auxotroph different from a prototroph?

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Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

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Q: You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes…

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Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

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Q: In the Meselson  experiment, would it have been possible to utilize N14 in the original bacteria and…

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