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Based on your observations recorded in the previous question, list the possible genotype(s) of strains 1, 2, and 3, if each strain only had one mutation within its/lac operon 3. Strain Possible Genotype(s) 1 2 3

Based on your observations recorded in the previous question, list the possible genotype(s) of strains 1, 2, and 3, if each strain only had one mutation within its/lac operon 3. Strain Possible Genotype(s) 1 2 3

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Using question 2 you can answer question 3 which I need help with
3. Based on your observations recorded in the previous question, list the possible genotype(s) of strains 1, 2, and 3, if each strain only had one mutation within its/lac operon. Strain Possible Genotype(s) 1 3.
Transcribed Image Text:3. Based on your observations recorded in the previous question, list the possible genotype(s) of strains 1, 2, and 3, if each strain only had one mutation within its/lac operon. Strain Possible Genotype(s) 1 3.
2. Complete the following table based on your B-galactosidase assay. Susstrute Mellow Color Change E. coli strain IPTG Water ONPG buffer Observation A 0.3 ml wild-type 0.4 ml 100 µl 10 µl Nettow 0.3 ml wild-type 0.4 ml 100 µl 10 µl 0.3 ml strain 1 0.4 ml 100 µl 10 µl Yelloce C 0.3 ml strain 1 0.4 ml 100 µl 10μ yellow. E 0.3 ml strain 2 0.4 ml 100 µl 10 µl Clear F 0.3 ml strain 2 0.4 ml 100 µl 10 ul Clear 0.3 ml strain 3 0.4 ml 100 ul 10 µl 0.3 ml strain 3 0.4 ml 100 µl 10 ul 0.3 ml negative 0.4 ml 100 µl 10 µl control (no E. coli) 0.3 ml negative 0.4 ml 100 ul 10 µl control (no E. coli)
Transcribed Image Text:2. Complete the following table based on your B-galactosidase assay. Susstrute Mellow Color Change E. coli strain IPTG Water ONPG buffer Observation A 0.3 ml wild-type 0.4 ml 100 µl 10 µl Nettow 0.3 ml wild-type 0.4 ml 100 µl 10 µl 0.3 ml strain 1 0.4 ml 100 µl 10 µl Yelloce C 0.3 ml strain 1 0.4 ml 100 µl 10μ yellow. E 0.3 ml strain 2 0.4 ml 100 µl 10 µl Clear F 0.3 ml strain 2 0.4 ml 100 µl 10 ul Clear 0.3 ml strain 3 0.4 ml 100 ul 10 µl 0.3 ml strain 3 0.4 ml 100 µl 10 ul 0.3 ml negative 0.4 ml 100 µl 10 µl control (no E. coli) 0.3 ml negative 0.4 ml 100 ul 10 µl control (no E. coli)
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Step 1

β-Galactosidasetest detects the presence or absence of Galactosidase enzyme in the bacterial strain. This enzyme. This enzyme is necessary for lactose metabolism in bacteria. 

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