This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution. Different cells and culture media absorb best at a specific, known, wavelength. In this case the amount of light transmission is
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This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution. Different cells and culture media absorb best at a specific, known, wavelength. In this case the amount of light transmission is related to the number of bacteria present. Optical Density is the value given to the measurement of turbidity. Environmental factors, such as temperature and pH, can disrupt the structure of enzymes, resulting in denaturation and making them unable to function.
The growth of microorganisms can be controlled in the laboratory by altering environmental factors, such as temperature, oxygen content, osmotic pressure, and pH.
write in your own words the Microbial Growth - Effects of pH
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- Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)? the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x the transmission electron microscope (TEM), with good resolution up to about 100,000x the scanning electron microscope (SEM), with good resolution up to about 1,000,000x the compound light microscope, with good resolution up to about 1,500x all of the above microscopes would be equally useful in visualizing the interior of organellesWhat is an analogy that can be used to help explain the risks associated with antibiotics? An analogy is a way of explaining an unfamiliar complex idea by using a comparison to a more familiar, less complex idea. For example, describing ATP as the "currency" of the cell. Describe the analogy in an easily understandable way, while maintaining scientific accuracy.choose a product of nanotechnology found at home. Discuss the purpose and how the technology is used. It is durable? It is reusable? How will you dispose of it if it is not usable anymore? Is it a potential health and/or environmental hazard? why or why not.
- A person is trying to extract a pigment found in the thylakoid membrane of a plant cell. Which would be the best option to use as an extraction buffer? Water An extraction buffer with detergent A nonpolar solvent A polar solventA biochemist grinds up a sample of plant roots, fungal mycorrhizae, and soil-dwelling arthropods. They then extract a mix of several polymeric molecules. Of the polymers found within the sample, which of the following molecules is least likely to be used as a component of a cell wall? Group of answer choices A. Chitin B. Glucan C. Starch D. CelluloseTwo wavelengths of light strike a living cell, one has a wavelength of 12 nm, and one has a wavelength 700 nm. Based on the electromagnetic spectrum, which one would be considered more dangerous to the cell? Why? (note: 1 m = 109 nm)
- Microscope You should always position the cells or tissues in the center of view before increasing magnification, because you know that the image will move right as you move the slide to the left. you know that the image will move up as you move the slide down you know that the center of field of view will be unchanged this is the best way to avoid breaking the slide with an objectiveFill in the blanks using the following terms: more, less, equal, in, out, zero, plasmolyze, swell, stay the same. 1. If plant cells are placed in a hypertonic solution which has _ solute and _ solvent than the cells, the net movement of water will be _ and the cells will _. 2. If plant cells are placed in a hypotonic solution which has _ solute and the _ solvent than the cells, the net movement of water will be _ and the cells will _. 3. If plant cells are placed in an isotonic solution which has a solution _ in concetration to the cell, the net movement of water will be _ and the cell will _.Dr. Rodo and is colleague Mr. Simms were studying the effect of a particular herbacide on the activity of plant cells. They want to observe the impact of the chemical on the molecular structures inside the cell. Which microscope would be the best tool for this research? a. dissecting microscope b. compound light microscope c. scanning electron microscope d. transmission electron microscope
- SELECT ALL THAT APPLY An atomic force microscope can be used to measure Group of answer choices Surface features on a nano scale Thickness of a thin film layer The surface of a cell An STM The surface of a self assembled monolayerHemidesmosomes and focal adhesions link the inside of a cell to a substrate. True Falseyou are looking at a slide in the laboratory and observe a cell that occupies one quarter of the field of view at high magnification. use your field diameter calculation from lab activity 4 to estimate the size of this cell.