you are looking at a slide in the laboratory and observe a cell that occupies one quarter of the field of view at high magnification. use your field diameter calculation from lab activity 4 to estimate the size of this cell. EXERCISE 4 Use of the Microscope2. Use the coarse adjustment knob to bring the threads intofocus. Find the area where the threads overlap.423. Rotate the nosepiece to select the low-power objective lens.4. Use the fine adjustment knob to focus through theoverlapping threads. After determining which thread is ontop, which is in the middle, and which is on the bottom,write your observations in the space provided.Color of top threadColor of middle threadColor of bottom thread.4Relationship Between Magnificationand Field DiameterAt scanning magnification, the diameter of the field of view islarge and most of the slide specimen is visible. As magnifica-tion increases, the field diameter decreases, because at higherpower the objective lens is closer to the slide and magnifies asmaller area. Figure 4.5 reviews the relationship between mag-nification and field diameter.Field diameter at scanning and low magnifications canbe measured using millimeter graph paper glued to a micro-scope slide. By aligning a vertical marking on the paper withthe edge of the field and then counting the number of mil-limeter (mm) squares across the field, you can determine thediameter (Figure 4.6). Knowing the diameter of the field ofview enables you to estimate the actual size of an object. Forexample, if the field diameter is 4 mm and an object occupiesone-half of the field, the object is approximately 2 mm wide.Figure 4.5 Magnification and Field Diameter Each circle on theslide illustrates the field diameter for a particular magnification; thecorresponding circle outside the slide represents that magnification.Scanning power40XLow power100XHigh power400XFigure 4.6 Calculation of Field Diameter Using Millimeter GraphPaper In this sample, the field is approximately 3.5 mm in diameter.1Field Diameter23Study Tip Field DiameterYou can demonstrate the relationship between magnification andfield diameter by curling your fingers until the thumb of each handoverlaps the index and middle fingers of the same hand. The spaceenclosed by the curled fingers of each hand forms the barrel of a"lens." Place these two "lenses" to your eyes, and, while sitting upstraight in your chair, look at this page. Notice that you can see theentire page with this "scanning lens." Now slowly bend forward untilthe "lenses" are just a few inches way from the page. In this "high-magnification" view, the field of view is much smaller, and you cansee only part of the page.Once you know the field diameter for one magnification-we call this lens A in the following formula-you can calcu-late the field diameter for other magnifications (lens B) usingthe formula shown below:Field diameter of lens BField diameter of lens A X total magnification of lens ATotal magnification of lens BQuickCheck Question4.1 What happens to the field diameter as magnificationincreases?

Question

you are looking at a slide in the laboratory and observe a cell that occupies one quarter of the field of view at high magnification. use your field diameter calculation from lab activity 4 to estimate the size of this cell. EXERCISE 4 Use of the Microscope2. Use the coarse adjustment knob to bring the threads intofocus. Find the area where the threads overlap.423. Rotate the nosepiece to select the low-power objective lens.4. Use the fine adjustment knob to focus through theoverlapping threads. After determining which thread is ontop, which is in the middle, and which is on the bottom,write your observations in the space provided.Color of top threadColor of middle threadColor of bottom thread.4Relationship Between Magnificationand Field DiameterAt scanning magnification, the diameter of the field of view islarge and most of the slide specimen is visible. As magnifica-tion increases, the field diameter decreases, because at higherpower the objective lens is closer to the slide and magnifies asmaller area. Figure 4.5 reviews the relationship between mag-nification and field diameter.Field diameter at scanning and low magnifications canbe measured using millimeter graph paper glued to a micro-scope slide. By aligning a vertical marking on the paper withthe edge of the field and then counting the number of mil-limeter (mm) squares across the field, you can determine thediameter (Figure 4.6). Knowing the diameter of the field ofview enables you to estimate the actual size of an object. Forexample, if the field diameter is 4 mm and an object occupiesone-half of the field, the object is approximately 2 mm wide.Figure 4.5 Magnification and Field Diameter Each circle on theslide illustrates the field diameter for a particular magnification; thecorresponding circle outside the slide represents that magnification.Scanning power40XLow power100XHigh power400XFigure 4.6 Calculation of Field Diameter Using Millimeter GraphPaper In this sample, the field is approximately 3.5 mm in diameter.1Field Diameter23Study Tip Field DiameterYou can demonstrate the relationship between magnification andfield diameter by curling your fingers until the thumb of each handoverlaps the index and middle fingers of the same hand. The spaceenclosed by the curled fingers of each hand forms the barrel of a&#34;lens.&#34; Place these two &#34;lenses&#34; to your eyes, and, while sitting upstraight in your chair, look at this page. Notice that you can see theentire page with this &#34;scanning lens.&#34; Now slowly bend forward untilthe &#34;lenses&#34; are just a few inches way from the page. In this &#34;high-magnification&#34; view, the field of view is much smaller, and you cansee only part of the page.Once you know the field diameter for one magnification-we call this lens A in the following formula-you can calcu-late the field diameter for other magnifications (lens B) usingthe formula shown below:Field diameter of lens BField diameter of lens A X total magnification of lens ATotal magnification of lens BQuickCheck Question4.1 What happens to the field diameter as magnificationincreases?

Accepted Answer

In order to visualize objects that cannot be seen with unaided eye, different types of microscopes…