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- DRAW IT The graph here showsthe pH difference across theinner mitochondrial membraneover time in an actively respiringcell. At the time indicated by thevertical arrow, a metabolic poisonis added that specifically andcompletely inhibits all function ofmitochondrial ATP synthase. Drawwhat you would expect to see forthe rest of the graphed line, andexplain your graph.Explain the organization of electron carriers in mitochondrial membrane.Explain why an intact, impermeable mitochondrial membrane is essential forATP synthesis.
- Assuming that 3 H+ are transported per ATP synthesized in the mitochondrial matrix, the membrane potential difference is 0.18V (negative inside), and the pH difference is 2 unit (acid outside, basic inside), calculate the largest ratio of ATP/(ADP)(Pi) under which synthesis of ATP can occur. (gas constant 8.3J/mole, F is 96,485 kJ/V, T in , Co +273)In phase II of ETS diagram and label the ATP synthase along with the inner mitochondrial membrane it is sitting in. Your diagram must show the intermembrane space and the mitochondrial matrix. Describe how it works step by stepDefine chemiosmosis and explain how a gradient of protons is established across the inner mitochondrial membrane.
- A new weight loss drug, Super Fat Melter, is introduced on the market but then quickly recalled after a few patients die. As it turns out, the pill makes the lipid bilayer of the inner mitochondrial membrane leaky to H+. What impact does this have on ATP production? Explain, with reference to the type of phosphorylation affected.Energy-consuming active transport is used to move protons in all of the following directions except: A. from the mitochondrial matrix, across the mitochondrial cristae, into the intermembrane space B. from the extracellular fluid, across the plasma membrane, into the Halobacterium halobium cytosol C. from the cytosol of plant cells, across the vacuolar membrane, into the central vacuole D. from the cytosol of skeletal muscle cells, across the plasma membrane, into the extracellular fluid E. from the chloroplast stroma, across the thylakoid membrane, into the thylakoid spaceDraw and label a lipid bilayer containing the large mitochondrial trans-membrane protein complexes representing complex I, II, III, and IV, and ATP Synthase. Make two more of these drawings. Label the first one mitochondrial electron source and using a different ink color, indicate the source(s) of electrons. Label the second mitochondrial energy source, and indicate the source of energy driving the electron transport chain. In the third, label the mitochondrial electron acceptor in its proper position. In this third drawing, also include ATP synthase (in its correct orientation-the spherical part is on the opposite side of the membrane as the H+ reservoir). Label your diagrams with the correct names for the membranes, ATP synthase, and to show where the protons (H+) are pumped to drive ATP synthesis.
- An important function of the inner mitochondrial membrane is to provide a selectively permeable barrier to the movement of water soluble molecules and thus to generate different chemical environments on either side of the mem- brane. However, many of the substrates and products of oxidative phosphorylation are water soluble and must cross the inner membrane. How does this transport occur?The ATP synthase F1 of mitochondrial inner membranes sticks into the ________ and the ________ conformation of the active site catalyzes ATP synthesis. A. matrix; loose B. intermembrane space; open C. matrix; tight D. intermembrane space; tight E. matrix; openCould the inner mitochondrial membrane carry out its functions in the coupling of electron transport and ATP synthesis if its lipid bilayer were readily permeable to hydrogen ions (protons)?