Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out)After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4.Why is there a need to gradually add the ammonium sulfate to the sample? Table 2.1.iSODUOImaes™table (grams per 1L sample).104070 80 905060100Grams of solid (NH4)2SO4 to be added to 1 L of solution176(242 314 390 472 561 657551376157117182251 325 405 491 5846852059188 260121337 4215116093061126195 270 351 4385334063130202281365457(NH 504)2s 6065135 210 | 29238167140 2193057070146228satu 80731529076

The experiment is about isolation of crude ovalbumin, which is a protein.

Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4. Why is there a need to gradually add the ammonium sulfate to the sample?

Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4. Why is there a need to gradually add the ammonium sulfate to the sample?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter8: Sampling, Standardization, And Calibration

Section: Chapter Questions

Problem 8.1QAP

See similar textbooks

Similar questions

Stock iron(II) solution (200Ug mL-1 Fe) ferrous ammonium sulfate hexahydrate mass= 0.1437g, transfer it to a 100 ml beaker. add 15 ml approx of water and 15m1 'approx of dilute sulphuric acid (2M H2SO.). then transfer FeII to 100 ml flask makeup to the mark with water. calculate the moles of ferrous ammonium sulfate hexahydrate solution in unit ug/mL.
Given the following data forMass of test tube, beaker and cyclohexane = 100.17 gMass of test tube and beaker = 84.07 gFreezing point of cyclohexane = 6.59 oCMass of weighing paper + naphthalene =1.080 gMass of weighing paper = 0.928 gFreezing point solution = 5.11oCKf = 20.8oC/mDetermine the followinga. mass of cyclohexane in g (2 decimal places); _____b. mass of naphthalene in g (4 decimal places); _____c. freezing point depression (2 decimal places); _____d. molality of solution (3 significant figures); _____e. moles of naphthalene (3 significant figures); _____f. molar mass of naphthalene, experimentally (3 significant figures); _____g. % error if theoretical molar mass of naphthalene is 128.17 g/ mole, USE ABSOLUTE VALUE (3 significant figure); ____
Concentration of AR Stock Solution (ppmppm) 21.22 Unrounded Rounded Concentration of AR Stock Solution (μMμM) 42.7461 42.7
I have an equation and the answer, however im not sure how to do the calculation. Please show how to. Answer is supposed to be 0.20 M m1v1=m2v2 (20mL)V=(13mL)(0.30M) V=0.20 M
Beaker 0.00200 M Fe(NO3)3, mL 0.00200 M NaSCN, mL total volume, mL 1 3.000 2.000 10.00 2 3.000 3.000 10.00 3 3.000 4.000 10.00 4 3.000 5.000 10.00 5 (blank) 3.000 0.000 10.00 In Solutions 1-4 you are adding successively larger volumes of 0.00200M SCN- to the Fe3+ solution and diluting to 10.00 ml. Calculate the final diluted molarity of SCN- in solution #1 Your answer should have 3 sig figs =
Read the Limit Test for salicylic acid in aspirin below then answer the question below. Remember: Aspirin = acetyl salicylic acid (ASA), and salicylic acid = SA Limit Test for salicylic acid: Extract powdered Aspirin tablets (i.e., active + excipient) containing 0.4g acetylsalicylic acid (i.e., active) with 4 mL of 95% ethanol. Add water to give a final volume of 100.0 mL and mix. Transfer 50.0 mL of the filtrate to a Nessler Cylinder and add 1.0 mL of acidified ferric ammonium sulfate solution. Mix and allow to stand. Prepare the standard solution used for comparison as follows: To another Nessler Cylinder add 3.0 mL of freshly prepared 0.01% (wt/vol) salicylic acid, 2.0 mL of ethanol, 1.0 mL of acidified ferric ammonium sulfate solution and sufficient water to give a total volume of 51.0 mL. Mix. A violet colour produced by the sample should not be more intense than that of the standard, used for comparison. Example of "Limit" calculations: Based on the above limit test, the…
A liniment contains 15 % (v/v) of methyl salicylate. How many mL of the liniment can be made from 1 pint of methyl salicylate? Volume(ml) x %(expressed in decimal)= mL of active ingredients
an aqueous hno3 solution contains 1.623 kg hno3/kg h2o and has a specific gravity of 1.304 at 20 degrees celsius. express the composition of the solution in: a. weight percent of HNO3 b. lbm HNO3/ft3 solution at 20 degrees Celsius c. molarity
(a) A solution of 20.91 % by weight H₂SO4, has a specific gravity of 1.150. What is the normality of this solution? (b) Water is added to dilute this solution to a solution of 3.03% by weight H2SO4 (SG=1.020). What is the new normality of the resulting solution and how were they mixed? (Assume 100 mL of the original solution was used.)
93.6g to ng and 93.6g to tons with the set up in the image possible show how to get the answer please
Convert 450 m2/kg into cm2/g. Need detailed explanation for solution.
Beaker 0.00200 M Fe(NO3)3, mL 0.00200 M NaSCN, mL total volume, mL 1 3.000 2.000 10.00 2 3.000 3.000 10.00 3 3.000 4.000 10.00 4 3.000 5.000 10.00 5 (blank) 3.000 0.000 10.00 In solutions 1-4 you are adding 3.00ml of 0.00200M Fe3+ to some SCN- solution and dilutiing to a final volume of 10.00ml. What is the new concentration of the Fe3+ due to dilution? Your answer should have 3 sig figs.