TROUBLESHOOTING PROBLEMS CAUSE TECHNIQUES A. Paraffin is too A. Choose a sticky or too hard paraffin with a lower melting point. B. Incomplete dehydration or clearing B. Clamp tightly. The block and blade should be tightly clamped in the microtome. C. Loose knife holder 2 C. Trim the block parallel to the other edge or re-embed them in molds so tissue removed from that the edge of D. Unparalleled block edges E. Small flecks of the blocks block is parallel to the knife edge. D. If there is sufficient tissue in the block, cut and discard ribbons until the holes disappear. CITY E. Soak the block in tap water for a few hours. RSH

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter5: Biomedical Electronics: Digital
Section: Chapter Questions
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The pictures on the second column are some of the problems encountered in improper techniques of tissue processing. Match the pictures on what causes (Column 3) them and on troublshooting techniques (Column 4) that fixes them.

The pictures on the second column are some of the problems encountered in
improper techniques of tissue processing. Match the pictures on what causes
(Column 3) them and on troubleshooting techniques (Column 4) that fixes them.
Luate
TROUBLESHOOTING
ANSWER
PROBLEMS
CAUSE
TECHNIQUES
A. Paraffin is too
sticky or too hard
A. Choose a
paraffin with a lower
melting point.
B. Incomplete
dehydration or
clearing
B. Clamp tightly. The
block and blade
C. Loose knife
should be tightly
clamped in the
holder
microtome.
C. Trim the block
parallel to the other
edge or re-embed
them in molds so
tissue removed from that the edge of
D. Unparalleled
block edges
E. Small flecks of
the blocks
block is parallel to
the knife edge.
D. If there is
sufficient tissue in
EDIFICA
the block, cut and
discard ribbons until
CITY
the holes disappear.
E. Soak the block in
tap water for a few
hours.
5
RST
2.
Transcribed Image Text:The pictures on the second column are some of the problems encountered in improper techniques of tissue processing. Match the pictures on what causes (Column 3) them and on troubleshooting techniques (Column 4) that fixes them. Luate TROUBLESHOOTING ANSWER PROBLEMS CAUSE TECHNIQUES A. Paraffin is too sticky or too hard A. Choose a paraffin with a lower melting point. B. Incomplete dehydration or clearing B. Clamp tightly. The block and blade C. Loose knife should be tightly clamped in the holder microtome. C. Trim the block parallel to the other edge or re-embed them in molds so tissue removed from that the edge of D. Unparalleled block edges E. Small flecks of the blocks block is parallel to the knife edge. D. If there is sufficient tissue in EDIFICA the block, cut and discard ribbons until CITY the holes disappear. E. Soak the block in tap water for a few hours. 5 RST 2.
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