True or False: An isolated bacterial colony supplies cells.to grow "pure culture" of the bacteria. True False

Q: A subculture is a a. colony growing beneath the media surface b. culture made from a contaminant c.…

A: We make subculture from a plate where many different colonies of bacteria are growing.

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Experiment :method of sterilization on glassware and media

A: Sterilization is a process by which all forms of life are killed, deactivated or removed from a…

Q: Why are the results of plate counts expressed as the number of colony-forming units, rather than the…

A: Microbes are small living organisms  which can not be seen through naked eyes. The study of these…

Q: Irue or False: An isolated bacterial colony supplies cells to grow a "pure culture" of the bacter…

A: A pure culture of bacteria is a population of bacterial cells growing in the absence of other…

Q: The number of bacteria in a culture every 2 hours and 30 minutes. If the initial number of Bacteria…

A: In the question, we are given: Generation time of bacteria: 2 hours and 30 min.  Initial number of…

Q: Which one of the terms below is NOT a stage in the typical growth curve of a bacterial cell under…

A: A typical growth curve of a bacterial cell under ideal condition in culture generally has four…

Q: True or False: both gram-positive and gram-negative bacteria have a cell membrane inside of their…

A: Microorganisms are those small organisms that are invisible to the normal naked eye and can be seen…

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: You begin with an overnight culture of approximately 108 organisms/ml and need a concentration of…

A: In an Overnight culture of the bacteria, their growth factor depends upon the type of bacteria we…

Q: When a medical technologist wants to determine if a clinical specimen contains a Mycobacterium…

A: Answer is a.) Acid -fast stain.

Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…

A: Option b

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: Describe how to make bacterial culture in different media. Explain different cultural techniques…

A: The predigested protein provides nutrition for the growth of bacteria. A culture medium must contain…

Q: The below  photograph shows a Gram-stained slide viewed using a light microscope set at brightfield…

A: Gram's staining is a technique used to distinguish between Gram negative and Gram positive bacteria.…

Q: Based on these results, which of the following statements is true regarding the effectiveness of…

A: A disinfectant is a chemical substance or compound that is used to inactivate or destroy the…

Q: Which one of the following is NOT considered a biohazard?            old bacterial cultures slides…

A: Biohazard is defined as any biological substance that can pose or cause a threat and harm to biotic…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: On a spread plate, you count 50 colonies. The plate was created from a 10^-2 dilution and 0.2 mL of…

A: Microbial culture refers to the method of letting the micro-organisms multiplying in the culture…

Q: Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the…

A: The wet mount is the most basic method of preparation, in which the specimen is placed on the slide…

Q: A bacterial sample collected from a patient is processed using a Gram's stain. After staining, the…

A: Gram stain is a test that looks for bacteria in areas where an infection is suspected, such as the…

Q: suffering severely due to alkaloid production and accumulation in the medium the

A: Plants have an important source of life-saving medications where it is used in two ways such as…

Q: All microorganisms have a similar range of living conditions.  true / false

A: Answer:  MICROORGANISMS = These are the microscopic organisms which cannot be seen by naked eyes.…

Q: If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were…

A: Dilution Factor is the factor by which the original solution is diluted. It may be expressed as the…

Q: chemically defined media  b.    complex culture media

A: Media is a growth medium of solid,liquid and semi solid type. Diffarent types of media are used for…

Q: typical labeled sketch of a bacterial growth curve in batch culture

A: Batch culture is a laboratory culture method in which bacteria has grown in large scale.  Here…

Q: Your lab partner is observing a Gram-stained slide of their unknown organism with 1000X total…

A: In high magnification (1000X) the oil emulsion is used. It is used when we use 100X objective lens…

Q: differential plate enrichment plate selective plate streak plate pour plate

A: Differential plate:  Differential media used to distinguish between two related microbes. It used to…

Q: When viable cell concentrations are too high to count on an agar plate, it is common to use larger…

A: The correct option is shown below.

Q: Based on the photo, describe the bacteria observed from the different staining technique. -…

A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…

Q: Which cellular structure is being detected in the motility test? bacterial ribosomes bacterial cilia…

A: Prokaryotes are characterised by the absence of nucleus and membrane bound organelles.

Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…

A: answer given below

Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…

A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…

Q: You partner stained bacterial cells and saw only the background and not the actual cell was stained.…

A: Staining technique is a method in which recognition and visualisation of structure ,  arrangement of…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: Colony formation can be observed in liquid media.  a) Trueb) False

A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…

Q: A SAB agar plate is exposed to air for 30 minutes and then incubated. The 10 colonies which grew on…

A: Sabouraud Dextrose Agar (SDA) is used for the isolation, cultivation and maintenance of…

Q: Which of the following words apply to the overlay that maintains proximity between virus and…

A:

Q: The raw product of an electron microscope is a color image of the object. True False

A: Introduction An electron microscope is a types of microscope where a beam of electron is used as…

Q: True or False: if an organism is multicellular, you can be certain that it is not a bacterium.

A: Multicellular: These are the organisms which consists of more than one cell  Examples include human…

Q: __________ indicates ________________ in the cell culture.

A: Cell culture is a  primarily techniques in life sciences. This is a common term that is  used for…

Q: s shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number…

A: CFU count is the technique of enumeration of microbial cells in a sample and it is one of the most…

Q: It is called a "Negative Stain" because the stain has a negative charge, and is therefore: repelled…

A: An acid dye has negative charge hence are able to bind to the cell structures that are positively…

Q: Describe the difference between batch and continuous culture techniques

A: Bacteria and fungi, for example, are extremely valuable to a wide range of industries.…

Q: Which of the following reports bacteria counts as colony-forming units? Turbidity Most probable…

A: Bacteria are microscopic unicellular organisms that can be grown in culture for the benefit of the…

Q: Define the meaning of Suspension cell culture

A: Cell culture is a media which is used for growth of the cells.

Q: You are reading culture plates today and identifying pathogens that grow from patient samples. Which…

A: The culture plates for growing microbes hold importance in understanding and examining the…

Q: Endospores are very resistant to heat, drying, radiation and chemicals. True False

A: Introduction An endospore is a non-productive structure which is dormant and tough and is produced…

Q: Below is a diagram of the serial dilution of a culture with 1 x 10° cells. Fill out the missing…

A: Colony-forming unit / mL= Number of colonies * Dilution factor * 1/volume plated in mL CFU/mL = 1 *…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.