Spread-plate method Solid agar Incubate Culture, diluted as needed Bacterial colonies 0.1-0.2 mL appear only on surface. Spread cells onto surface of pre-poured solid agar. Pour-plate method 0.1-1.0 mL Melted cooled agar Incubate FIGURE 4.18 Spread Plates and Pour Plates Some colonies appear on surface; many are below surface. These techniques can be used for plate counts. Add melted cooled agar and swirl gently to mix.
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Why are the results of plate counts expressed as the number of colony-forming units, rather than the number of cells?
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- Need help Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.A culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?
- You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium, slant agar tube, and broth tube for further study and identification Question from the picture above (pure colonies white pinhead size) How many pure colonies of microorganisms are in the petri dish sample?procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantprocedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to broth
- Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!Need help Please help me in this. Minimum Inhibitory Concentration (MIC) determination of Citrus Disinfectant on 3 microorganisms:Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Please provide me with some information for my introduction (Organisms used in experiment ( family they belong to, cellular morphology: size, shape, Gram stain, flagella, biofilm?, colony morphology: size, shape margin, color; habitat and growth conditions- where is it normally found in the environment and /or humans, optimal temperature, atmospheric requirements; isolation and identification - biochemical test results, special media for isolation and importance of organism, medical significance and diseases it causesA. Observation of growth characteristics on selective and differential media Mannitol salt Agar Bacteria Growth (+/-) Describe growth characteristics Interpretation S. aureus E. coli P. aeruginosa M. luteus
- PLEASE ANS ASAP 7. Typhoid fever is caused by a species of: a . Escherichia b. Campylobacter c. Shigella. d. Salmonella 8. At the end of the Gram -staining procedure , Gram -positive bacteria will be: a. blue to purple b . pink to red c. orange. d. green 9. Which one of the following types of culture media is selective and differential? a. Phenylethyl alcohol agar b . Blood agar c. Thayer-Martin agar d. MacConkey agar 10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a.…procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies were counted. How many bacteria (CFU/ml) are in the sample? Thank you