Question 5Two different types of mastermixes were used to idetify the GMO food. What was the purpose of using plant mastermix?a. to show that both samples are of plant source and PCR worked for both mastermixesb. None of themC. Both of themd. to show that both samples are not of plant source and PCR worked for both mastermixes Question 24If you have the following primer sequence what would be its Tm?5 GAT TAC TTG GGC AAG GCC GA 3Ca 62Cb.72Oc 65d. 60

GMO Foods (Genetically modified foods) are produced from organisms (plants, animals and…

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 1. Although we will not be doing a gel electrophoresis, data from a gel digest of a Bacillus anthrax plasmid is provided so you can do a DNA map. The Bacillus anthrax plasmid is 4000bp (4Kb) long. Note the origin position as well as the reference molecular weight markers on the gel. Two restriction enzymes, A and B, were used to obtain two individual digests, A and B. They were combined to produce the third digest. The restriction enzyme fragment pattern for the digest of Bacillus anthrax plasmid Determining the Number of Fragments How many fragments were produced by enzyme A? How many fragments were produced by enzyme B? How many fragments were produced by the combined digest (A and B)? Fragment Size Fragment size is relative to molecular weight, and must be determined by comparing the fragment distance to the molecular weight markers. The fragment size has been provided on the gel pattern for this exercise. To make a map you must determine the relative positions of the…
Question 6 In the first part of the experiment, there were only a few colonies on the streptomycin positive plate. In the second part, there are a large number of colonies on the streptomycin positive plate that was inoculated with the antibiotic resistant strain. What is the most likely explanation for this difference? 1. The antibiotic resistant strain was composed of a large number of individuals with resistance to streptomycin 2. The exposure to streptomycin caused a mutation in a few individuals
QUESTION 40 Under what situation would you expect haplotype frequencies to remain unchanged from one generation to the next? a. the recombination rate is zero b. the coefficient of LD is 0.5 c. the two loci are in linkage disequilibrium d. crossing over between two loci occurs regularly
Question 8 In a clinical trial of Bacillus Chalmette –Guerin vaccination 1012 children were vaccinated and 20 died and 950 were not and 20 died. Assuming this was a randomized clinical trial a) What is the relative risk of dying? b) Is the vaccine protective against dying composed with the place to (control)?
QUESTION 2 2.1 Recombinant plasmids are often used to produce therapeutic proteins. Elaborate on the steps you would follow to generate a recombinant plasmid that can be used in future toexpress a therapeutic protein of your choice. In your answer, include the important reagents used and their functions. 2.2 Thoroughly explain at least three different techniques you would use to confirm successful recombination in 2.1. In your answer, outline the principle behind each technique. 2.3 With supporting points, outline the Polymerase chain reaction (PCR) technique you would choose to amplify a desired gene from a Retrovirus.
QUESTION 5You are asked to recommend an antibiotic to treat potential pathogenicityt in an animal. Describe a method which will allow you to make this recommendation.
QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology. QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes.
QUESTION NO. 1 Antisense nucleic acids A. complementary to mRNA would enhance translation . B. could result if a gene is inserted downstream of a promoter but in opposite direction to normal. C. can have no clinical uses. D. react only with DNA. E. are necessary for recombinant DNA technology.QUESTION NO. 2 There is a window in which the effect is primarily on viral replication since AZT is much less effective at competing with dTTP for incorporation by cellular DNA polymerases because of the proofreading ability of DNA polymerases. Proofreading activity co maintain the fidelity of DNA synthesis A. occurs after the synthesis has been completed. B. is a function of 3' co 5' exonuclease activity intrinsic to or associated with DNA polymerases. C. requires the presence of an enzyme separate from the DNA polymerases. D. removes mismatched bases in the interior of the chain. E. does nor occur in prokaryotes. QUESTION NO. 3…
QUESTION 27 Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines? A. Immunohistochemistry B. Western blotting C. Polymerase chain reaction (PCR) D. Immunocytochemistry
Question is complete please explain why option B is correct why not D?
Question 9 What is the most likely reason that there were so few bacterial colonies growing on the streptomycin-positive plate in the first part of the experiment? 1. Streptomycin in the medium caused deleterious mutations in the bacteria. 2. Only a few individuals in the original bacterial culture had the mutation that conferred resistance to streptomycin .

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Two different types of mastermixes were used to idetify the GMO food. What was the purpose of using plant mastemix? a. to show that both samples are of plant source and RCR uoruo