Two solutes have an unadjusted retention of γ = 1.06. How many plates are required to give a resolution of 1.5? Of 3.0? While we did not discuss this in class, the number of plates can also be expressed as N = L/H where H is plate height and L is the length of the entire column. If the plate height is 0.5 mm in gas chromatography, how long must the column be for a resolution of 1.5? If γ = 1.06 and H = 3 microns in liquid chromatography, what length of column in cm gives a resolution of 1.5?

Two solutes have an unadjusted retention of γ = 1.06. How many plates are required to give a resolution of 1.5? Of 3.0? While we did not discuss this in class, the number of plates can also be expressed as N = L/H where H is plate height and L is the length of the entire column. If the plate height is 0.5 mm in gas chromatography, how long must the column be for a resolution of 1.5? If γ = 1.06 and H = 3 microns in liquid chromatography, what length of column in cm gives a resolution of 1.5?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.2QAP

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